FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1913-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1913-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590322
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA19820.12462884874886973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT2650.057.455861
TTACGTG2950.051.612892
GTATAGG7300.045.6873361
TATACCG2150.043.839525
GTCGGTA2650.038.3015145
CGTGTTG4000.038.0645075
TACGTGT4400.036.251913
TACCGAG3050.033.278357
GCGAAAG6850.032.8119471
ACTCGTT1402.7478789E-531.071112145
CCTATAC7550.029.7697833
CGAAAGC7350.029.5933952
GATCCGT1000.006073957329.001536
ATACCGA3251.8189894E-1229.001536
CCGACTA4700.027.767424
GTTGACA13400.027.0536651
GTAACGG2456.440132E-826.6340581
CACCTAA20600.026.0450612
ACACCTA21300.025.1891211
TAACGGG3001.7078492E-824.167942
Produced by FastQC (version 0.11.5)