FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1913-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1913-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842330
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC9960.11824344378094095No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGCG604.944634E-448.333332145
TGTATCG907.481436E-540.277786
GTACTGA5500.039.5454566
AGCGAAA3500.037.2857131
GGTACTG5000.034.85
CAGGTAC5700.033.070183
GCAGGTA5950.031.6806742
ATCGTAG950.00472687630.5263182
ATACACG1204.0575082E-430.2083346
GCGAAAG4600.029.9456542
ACCCGTC2252.8159775E-828.9999987
CGAAAGC4900.028.1122463
ACTGATC6250.027.8399988
GACGGTA1050.00770777227.6190477
TATACTG3255.0931703E-1126.769235
TACTGAT7400.026.4527027
CCTAACG1100.00967073426.3636382
GTATAAG2259.1046604E-725.7777771
TACCCGT2001.0652084E-525.3756
ATCGTGC1450.001226282725.09
Produced by FastQC (version 0.11.5)