FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1912-d12-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1912-d12-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713141
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACT750.001483114738.666594
GTATATA3400.034.124761
TTAGCGA1102.4354793E-432.9544834
TTATACT2802.6193447E-1028.4820884
TATACTG2603.7161954E-927.8845625
ATATAGG2405.2672476E-827.1874493
TAACCGT1409.991883E-425.8928075
ATATCAC2559.47839E-825.5881843
GTTGACA6000.025.380291
AAGGGCG1450.00122611224.999955
ACATGGT6400.023.7890175
CCGACTA1902.1923399E-422.8946934
AGCGAAA2552.7003825E-622.749841
CCATCGT1600.002174566322.6562083
GGTCCTA8950.021.061419
GCGAAAG3106.2346953E-721.0498222
TATACAT4851.6370905E-1120.9277945
CCTATAT1750.003653300520.7186051
ATATATC3551.0137228E-720.4224973
GTCCTAT8950.020.255621
Produced by FastQC (version 0.11.5)