FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1912-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1912-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences864231
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT10640.12311523192294653No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG8980.10390740438609586No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA8950.10356027497277927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG3500.049.7142642
AGCGAAA3250.049.0797421
CTATACG604.944715E-448.3333173
TATACTG7750.038.3548245
TACACGT1102.4358829E-432.9545335
TTATACT7500.032.8666534
CGAAAGC6100.028.5245783
ATGCCGG1855.8149544E-627.43242145
AAGGTCG3453.6379788E-1227.3188299
ATACTGT7050.025.709216
GTTATTA2601.143726E-725.0975971
GTAATAC2051.2900042E-524.756093
CCCTTAT9700.024.6663651
CCTTATA9700.024.6649382
GTAGAAA10800.024.1666582
TGGCGAT2101.5547566E-524.166656145
CTAGTAG1801.5200565E-424.1666563
TACACCG1500.001494112524.1666565
CCTACGG2202.228358E-523.0681723
GGATAGC3503.4997356E-922.7857065
Produced by FastQC (version 0.11.5)