FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1912-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1912-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121865
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAGAAAAGAGGAGGGATGCCTGTCTGGCTTAGTTGGTAGAGAATATG5320.4365486398884011No Hit
CCCCTAAATGCATACTTTAGAAAAGAGGAGGGATGCCTGTCTGGCTTAGT2790.22894186189636073No Hit
AAAGCAGGGAGTGGGAGGCAGAGGGAGAAGCAGGCTCCATGATGAGCAGG2760.22648012144586221No Hit
GCCTTACACCCAGGAAACTGCAGGGGAAAAATTTGATAGTCCAAAAAGTA2360.19365691543921554No Hit
GAGTGGGAGGCAGAGGGAGAAGCAGGCTCCATGATGAGCAGGGAGCCCAA1970.16165428958273498No Hit
GCATTACATCCAGTGAACAAATTATATTGCCTTACACCCAGGAAACTGCA1890.15508964838140565No Hit
ACATATAGTGCCAATTTGGATCAAAAGTCTTTAATCTCTTTCTCTCCTAA1750.14360152627907932No Hit
GAATATGATTCCTGATCTCAGGGTCTTGAGTTCAAGCCCTACCCCGGGGC1740.14278094612891315No Hit
GGCCTGAGAAATAAATCTATACCCCTAAATGCATACTTTAGAAAAGAGGA1730.14196036597874695No Hit
TCCCAGGACCTTGGGTTTATGAGATGCTTAACCGAATAGGCCACCCAGGC1700.1394986255282485No Hit
AAGCAGGGGAGTGAGAAAAGTACATGTTTTGTTTTAATAATCTGCTAAAA1560.12801050342592213No Hit
GAAATAAATCTATACCCCTAAATGCATACTTTAGAAAAGAGGAGGGATGC1510.1239076026750913No Hit
AAGCAGGCCTGAGAAATAAATCTATACCCCTAAATGCATACTTTAGAAAA1470.12062528207442663No Hit
GTGGGAGGCAGAGGGAGAAGCAGGCTCCATGATGAGCAGGGAGCCCAATG1440.11816354162392811No Hit
AAATGCATACTTTAGAAAAGAGGAGGGATGCCTGTCTGGCTTAGTTGGTA1400.11488122102326344No Hit
GCCCAATGCAGGGCTCGATCCCAGGACCTTGGGTTTATGAGATGCTTAAC1400.11488122102326344No Hit
AGCAGGGAGTGGGAGGCAGAGGGAGAAGCAGGCTCCATGATGAGCAGGGA1390.11406064087309728No Hit
AGTAGAAACAAGGCGTATAAATTTACTAAGAACATCAAGACCTCTAAGTG1370.11241948057276495No Hit
TGTCTGGGCTGTTACTCCGTGCTGTTCACTTCTAGGCGTCAAAGCAAATC1330.10913715997210027No Hit
ATCATGACCTGAGCCGAAGACAGACGCTTAATGACTGAGTCACCCAGGTG1330.10913715997210027No Hit
ATGATGAGCAGGGAGCCCAATGCAGGGCTCGATCCCAGGACCTTGGGTTT1280.10503425922126944No Hit
GGATAACTCAGCTTCAAACAGGATTGATAGATTCTTTCTACCTTTTGTAG1280.10503425922126944No Hit
CATCATAAAGAGTCAAGGCTACTCATAAACCTTAAGCCAGCAGATGTAGC1250.10257251877077093No Hit
AAATAAATCTATACCCCTAAATGCATACTTTAGAAAAGAGGAGGGATGCC1240.10175193862060478No Hit
ATGCAGGGCTCGATCCCAGGACCTTGGGTTTATGAGATGCTTAACCGAAT1220.10011077832027243No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTCGG100.007083277145.02
CGGGATA100.007083277145.06
TCTCCGC100.007083277145.0145
CGGAGCG100.007083277145.06
AGCGAAA2400.0129.895841
GCGAAAG3250.095.923072
GATAACT554.3619366E-879.0909042
CGAAAGC4050.076.975313
TAGGTTG300.001931650772.500017
ATAACTC607.972449E-872.500013
CTCTCGT451.1896712E-464.44444145
AGTAGAA2400.063.4375081
GCAATAG350.00355899462.1428573
CAATAGG350.00355899462.1428574
ATACAGC350.00355899462.1428576
TGCCGGG350.00355899462.142857145
ACAAGGC1800.060.4166688
AATGGCC400.00603819254.3758
AAGGCGT701.6871349E-551.7857139
AAGGCTC604.927888E-448.333346
Produced by FastQC (version 0.11.5)