FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1912-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1912-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771508
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT9090.1178212021132639No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA8990.11652503927373403No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG8060.10447072486610637No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC7840.1016191666191407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1550.074.838181
TATACCG300.001935131172.504185
TTACGTG1650.070.307082
ACGTGTT2150.057.3288884
AAGTCGA750.00148322838.666391
TATACTG6150.037.7257545
CGTGTTG3250.035.6943665
GGATAGC1956.9831003E-933.4634675
GTTGACA5350.032.5231321
GGTCGTT900.00362669632.2240837
AAGACGA1601.8755381E-631.720582
TACGTGT3900.031.6043853
GTTATGT5550.031.3511281
ACGTCCG1402.7460039E-531.0712059
ACGACTC1652.3844223E-630.759355
TATAACG1204.055824E-430.2100775
CGACTCC1703.009187E-629.8546646
GACGACT1703.009187E-629.8546644
CCGACTA1952.6046837E-729.7453044
GTAGTAC1000.00607189729.0016733
Produced by FastQC (version 0.11.5)