FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1912-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1912-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362877
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT85162.3468007065755065No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG67031.847182378602116No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT65931.8168690768497315No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG61701.7003006528382894No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA54761.5090512763277915No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT53781.4820448802211217No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC42671.1758805325220392No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA38411.0584853820991686No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA36711.0116375521182108No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG36160.9964809012420186No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA29760.8201126001372365No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA27510.7581081192800866No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG24510.67543547813722No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC23480.6470512046781691No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG22560.6216982613943567No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC22300.614533299161975No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG21880.6029591294019736No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG21450.5911093841714962No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC20940.5770550351772088No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG20650.5690633465333984No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT20100.5539066956572062No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG19840.5467417334248245No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT19800.5456394315429195No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA18750.5167040071429162No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA17480.48170592239243604No Hit
GGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAA17010.46875387528005363No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG16530.4555262526971949No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT16180.4458811112305272No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTC14410.39710425295623586No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA13710.3778139700229003No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTT13580.37423148890670943No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG13370.36844440402670875No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTA13290.366239800262899No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT13250.36513749838099413No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC12470.3436426116838488No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT11880.3273836589257517No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG11590.3193919702819413No Hit
GTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGA11450.31553391369527417No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACT11450.31553391369527417No Hit
ATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGG11410.31443161181336926No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT11110.3061643476990826No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG10510.2896298194705093No Hit
ATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAA10480.28880309305908064No Hit
TCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACT10380.2860473383543184No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC10280.28329158364955614No Hit
GGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGA9950.27419759312384084No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC9900.27281971577145975No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT9820.27061511200765No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA9530.26262342336383954No Hit
CCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCTCAG9500.26179669695241087No Hit
ATGCAATAAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCAT9260.2551828856609816No Hit
CTCTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCT9210.2538050083086004No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG8660.23864835743240823No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA8560.235892602727646No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA8450.23286127255240757No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGC8360.23038109331812157No Hit
GCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTT8320.22927879143621668No Hit
GTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCC8320.22927879143621668No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG8130.22404285749716846No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATC7940.21880692355812026No Hit
TGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTT7890.2174290462057391No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA7760.21384656508954825No Hit
GTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAAT7680.21164196132573845No Hit
TTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATG7590.20916178209145248No Hit
CTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAG7520.20723275379811892No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA7470.2058548764457378No Hit
GGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTG7430.20475257456383295No Hit
CTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCT7350.20254797080002315No Hit
GGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATAT7310.20144566891811827No Hit
ATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAAAATGCAATA7210.19868991421335605No Hit
TTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACA7170.19758761233145114No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT6940.19124937651049803No Hit
AAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATGGCCTCCATCAT6930.19097380104002182No Hit
GACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACACATCAG6870.1893203482171645No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG6800.18739131992383096No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA6790.18711574445335472No Hit
AGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGAT6790.18711574445335472No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT6740.18573786710097362No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGA6720.18518671616002116No Hit
TTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGTC6710.18491114068954495No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG6650.1832576878666876No Hit
GGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCAT6570.18105308410287785No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCA6570.18105308410287785No Hit
GAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAAAAT6560.1807775086324016No Hit
CTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGT6550.1805019331619254No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTC6510.1793996312800205No Hit
CAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA6490.17884848033906806No Hit
GTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTAT6430.17719502751621072No Hit
GCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAGGCGAATCAATA6210.17113236716573385No Hit
GATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGA6040.16644758416763805No Hit
GCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGC5990.16506970681525696No Hit
CCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACAG5980.16479413134478074No Hit
CAATGAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCT5850.16121165022858985No Hit
TCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCTCA5840.16093607475811364No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG5820.16038492381716118No Hit
TTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATA5810.16010934834668497No Hit
ATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTG5790.15955819740573252No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAG5740.15818032005335142No Hit
ATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAA5720.15762916911239896No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGT5660.15597571628954163No Hit
CCCCCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAA5640.15542456534858917No Hit
TAAAAGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACTGGAGA5540.15266881064382698No Hit
CCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGGGAGGTGG5540.15266881064382698No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGAT5520.15211765970287452No Hit
TTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAA5500.1515665087619221No Hit
ATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCA5420.1493619049981123No Hit
GAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGA5370.1479840276457312No Hit
ACTTTACTTTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATT5330.1468817257638263No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC5260.14495269747049275No Hit
AACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAG5190.1430236691771592No Hit
ATGGAAGACTTTGTGCGACAGTGCTTTAATCCAATGATTGTCGAGCTTGC5070.13971676353144452No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA5040.13889003712001588No Hit
GATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTC5010.1380633107085872No Hit
TGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATC4890.13475640506287254No Hit
ATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCC4840.13337852771049144No Hit
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATA4790.13200065035811034No Hit
ATACTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACAC4790.13200065035811034No Hit
GAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATACTGACAAA4710.12979604659430055No Hit
CCCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACA4650.12814259377144321No Hit
TCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGA4600.12676471641906212No Hit
ATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTC4580.12621356547810966No Hit
GGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCG4540.12511126359620478No Hit
TTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAG4440.12235550889144256No Hit
GAGTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGTGGAGATG4340.11959975418668034No Hit
ACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGG4310.11877302777525166No Hit
CCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACA4260.11739515042287056No Hit
ATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCT4230.11656842401144189No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAA4190.115466122129537No Hit
ATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAG4180.11519054665906078No Hit
CAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAG4170.11491497118858456No Hit
ACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGC4130.11381266930667969No Hit
ATGAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGC4120.11353709383620345No Hit
ATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGA4060.11188364101334612No Hit
CTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACTG4040.11133249007239368No Hit
GCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA4030.11105691460191744No Hit
CTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATA4020.11078133913144123No Hit
GATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAAAATGCAAT4000.11023018819048878No Hit
GAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCC3990.10995461272001257No Hit
CTGCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCAT3960.10912788630858389No Hit
CTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATGGCCTCCA3900.10747443348572656No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACC3880.10692328254477412No Hit
ATCTCGCACTCGCGAGATACTGACAAAAACCACTGTGGATCATATGGCCA3880.10692328254477412No Hit
CGCGAGATACTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAA3850.10609655613334547No Hit
GAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAA3850.10609655613334547No Hit
AGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACT3840.10582098066286923No Hit
GGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAAGA3810.10499425425144057No Hit
TTGTGCGACAGTGCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACA3800.10471867878096436No Hit
CAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG3650.10058504672382101No Hit
GTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCA3640.1003094712533448No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGA100.007089236144.998496
AACGGAG100.007089236144.998497
ACGGAGC100.007089236144.998498
CATAACG151.2294156E-4144.998494
ATAACGG100.007089236144.998495
AGCTACC100.007089236144.998496
GCAGGTA8050.0114.3934562
CAGGTAC8500.0109.175323
GGTACTG8500.0109.175325
GACGTCG203.8642113E-4108.748868
TATTAGT700.0103.584621
AGTAGAA14450.0103.369571
AGCAGGT9250.099.5532151
AGCGAAA1850.097.985451
GTACTGA9600.096.665666
ACTGATC9700.095.6691068
GGTCAAA4700.095.6504751
CTGATCC10050.092.337349
TACTGAT10200.090.979447
CCCGACG259.382285E-486.999095
Produced by FastQC (version 0.11.5)