FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1911-d12-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1911-d12-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663250
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG907.476708E-540.280525
AGCGAAA2151.2732926E-1137.0983471
GCGAAAG2800.033.6630062
CTAGTAC1555.4951168E-528.066433
GCACCGT1358.080171E-426.8516588
GCTATAC1100.00966607126.3654333
GTATATA3652.1827873E-1023.8390391
CGAAAGC3452.9576768E-923.1175163
CCTATAG1902.191076E-422.8962973
TATACTG3503.4924597E-922.7872665
GTATTAT7400.022.5370181
TAAGACC3355.487891E-821.6432654
CTATACA2353.7048074E-521.5972164
TCCTATA3406.418668E-821.3249822
ATACTAG2755.1998322E-621.0939351
TAGACAG5251.8189894E-1220.7156965
GTACTGT3157.269409E-720.7156966
TAGTACT2104.3100418E-420.7156964
TTATACT3901.216722E-820.4501114
GTATAAG3208.450206E-720.393551
Produced by FastQC (version 0.11.5)