FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1911-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1911-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712882
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3650.043.7016681
CGAAAGC4000.043.4999773
TGTACCG1201.9723848E-742.2916455
GCGAAAG4450.035.8426782
GTACCGC1753.7726277E-628.9999896
CCGACTA1306.4811285E-427.88464
CCTATCG1306.4811285E-427.88467
TACCGCC2104.983085E-727.6190327
GTGTAGG4250.027.2960191
TAGGGTG3255.0931703E-1126.769225
CTATCGT1550.001807987123.3870858
TATCGTC1550.001807987123.3870859
TAGTACC3452.9613147E-923.1159324
GCACCGT2552.7052847E-622.7450876
GTCCTAT7050.022.6256891
GTATAGG1650.002600140421.9696883
GTACTAC1700.003090683421.3250161
GTTAGCC1700.003091941421.323523
TAGACTG5550.020.900895
GTCTAAC2104.3104324E-420.7157291
Produced by FastQC (version 0.11.5)