FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1911-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1911-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845887
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG11540.13642484161596052No Hit
GTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGT10570.12495758889780786No Hit
GTCTTCCGACTGGGCCTTTCGCTTAATTTGATGCCTGGCAGTTCCCTACT9480.11207170697740952No Hit
GATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCT8970.10604253286786533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG2700.048.332419
GTCATAG4450.030.9562932
CGAAAGC4800.030.2095413
TATACCG1204.0579776E-430.2077549
AGCGAAA4400.029.6637861
GTGATCC4700.027.7654278
CATAGTG5100.025.5892584
AGTGATC5500.025.0449757
ATACACG2151.8647188E-523.6055953
GCGAAAG5600.023.30452
GTCCTAT6000.022.9619641
GTATAAG3055.330039E-721.3968261
GTGCAAG4251.4661055E-920.4738271
ATAGTGA8150.020.460945
GTATTAC2505.9658385E-520.3032111
GTATCGG2505.9658385E-520.3032111
TAGTGAT7700.019.7735186
GTCATAC2307.952092E-418.9160351
ATAAGAC3852.3829489E-718.8319233
GAATAGT5509.640644E-1118.4574641
Produced by FastQC (version 0.11.5)