FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1911-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1911-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666900
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG951.02854734E-438.157745
AGCGAAA2950.036.867021
ATACCGC2651.382432E-1030.0942156
TACACCG1358.080071E-426.851745
ACCGTGC1907.1509858E-626.7104198
CGATAAC1100.00966951326.363532
GCGAAAG4500.024.1665692
CGAAAGC4550.023.9013
GTGTAGT3402.4992914E-923.4575441
CAGCGCG1650.002600022821.9696049
CTTGTAC3004.550402E-721.749913
GTGCTAA3355.487709E-821.6433241
GGACCGT2053.664776E-421.2194256
CTAAGCT2806.0854763E-620.71424
AGTACTA2455.110891E-520.71425
GTATTAG2959.548548E-619.662411
TACCGCC3701.5679325E-719.5945137
TATTAGG3451.7403054E-618.9129642
GTCCTAG5801.0913936E-1118.7513281
CTGTTCG4303.7354766E-818.5464349
Produced by FastQC (version 0.11.5)