FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1911-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1911-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513730
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG451.1933077E-464.444449
GCGAAAG1101.6734703E-952.7272762
AGCGAAA1401.4122634E-841.428571
CGAAAGC1751.0075746E-733.1428573
CCGACTA1255.1503954E-429.04
CAATAGG1306.478625E-427.8846171
GGTATAT3005.857146E-1026.5833341
CTAATAC1851.8289802E-423.5135123
ATAGGAC3102.4121618E-823.3870963
CTCTATA2802.3380198E-723.3035722
TCGATTA1902.1911522E-422.894737145
TCTGGAC3901.2159944E-820.4487193
GAGTGCC2155.0515356E-420.2325579
GTATATA5750.020.1739121
GTATTAA8400.019.851191
GTATTAG2608.06756E-519.5192321
GATATAT5204.3655746E-1119.5192321
ATAGGGT2256.861457E-419.3333324
TATATAC5254.9112714E-1119.3333323
CACCGAC1900.00588407319.0789472
Produced by FastQC (version 0.11.5)