FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_1910_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1910_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6029
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC160.2653839774423619No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT80.13269198872118096No Hit
CTTTAATACCTCACACTTCTCTCAATGATCCCCCATCCAGACAGAAGATC80.13269198872118096No Hit
TTCTAGTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAG70.11610549013103333No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA70.11610549013103333No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAG100.006917387145.02
CAATGCA100.006917387145.04
AACCCAC100.006917387145.01
CCACTGA100.006917387145.04
Produced by FastQC (version 0.11.5)