FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1910-d12-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1910-d12-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991145
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG3650.037.7430882
GTATACG2350.037.026441
CGAAAGC4150.033.1957243
TATACGC900.00362663432.2250945
AGCGAAA4350.031.6710851
TAGACGT1050.007705067827.6215085
GTAGACG1100.00966734626.3659844
TACGTAG2507.823837E-826.1023254
TCCAACG2051.2893588E-524.7583032
TATACGT3001.7054845E-824.168822
TATACTG5850.023.5491075
CTGGCGA1550.001808071423.3889
TATATAC6000.022.9603793
TATAGCC2852.77636E-722.8967763
TATGGTG3004.5509478E-721.7519385
ATACGTA3355.49353E-821.6437193
GTATTAT9800.019.9772781
ATTAGGG3701.5683145E-719.596343
TATAGAG4102.1644155E-819.4529515
GTATATA5605.456968E-1219.4223521
Produced by FastQC (version 0.11.5)