FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1910-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1910-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences615659
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG6660.1081767666841547No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG6530.10606520817530483No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT803.7312537E-545.316031
GCGAAAG2250.041.892152
AGCGAAA2100.041.4317971
GTTATGT3900.033.464141
TAGCATG5950.032.9017222
CGAAAGC2900.032.502533
TATACTG2900.032.502535
GTTGACA4750.032.055131
TAGGGTA950.004724130.5286925
GTATACT1255.149221E-429.0022581
GCATGTT7000.027.9664654
TTACGTG1306.477148E-427.8867862
ACATGGT5550.027.4345665
CTACTGA8050.027.0207396
GCTATAT1358.0754916E-426.8539411
TCTAACC1409.985678E-425.8948733
GTAGCAT9950.025.5044981
TATATAG2301.1012144E-625.2193575
GTAATAT2601.1423981E-725.0981061
GTAATAC1450.001225351225.0019453
Produced by FastQC (version 0.11.5)