FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1910-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1910-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779550
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1051.1077645E-955.2443961
AGTACGG450.00963888448.332655
TTACGTG1307.348717E-944.6147542
TATACTG4400.036.2494855
AGCGAAA2653.6379788E-1232.8339351
CTACTGA8800.030.4825236
GTAATAT1952.6034468E-729.7469841
GTATATA2752.1282176E-1029.0033111
GTAGAAA4350.028.3329332
CAATTCG2507.8271114E-826.099632145
CGTGTTG2259.104315E-725.7774125
TACTGAA9350.025.5878737
GCGAAAG4300.025.290342
ACGTGTT2401.5975238E-624.1663254
TACACCG1801.5200122E-424.1663255
ACCGTGC1550.001807607623.3882648
GGTTATA1902.1908723E-422.8973481
CAACCCG1952.614277E-422.307377145
ACCTCTA5200.022.3073777
TAACCTA2303.1412186E-522.0649055
Produced by FastQC (version 0.11.5)