FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1910-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1910-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185138
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT26350.22233697679088846No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA16930.14285256231763727No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC14310.12074543217751857No Hit
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC12540.10581046257904143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1600.086.100361
GCGATTA300.001935889472.49945145
TTACGTG2100.065.600282
ACCGTAC604.9435825E-448.3370441
CGTGTTG3350.041.1225625
ACGTGTT3500.039.3601654
GCGAAAG3600.036.2527851
GCACCGT12350.035.812476
CTAGTAC1251.2646189E-534.8026733
ACCGTGT12850.033.8518878
CCGTGTT13250.033.3771069
GTTATGT7450.033.0897941
GTACTAG1102.4353218E-432.9570771
AGCACCG13500.032.7617765
TATACTG6650.030.528665
CACCGTG15500.028.5320437
GTATTAG2802.6375346E-1028.484331
GTTGGCC16150.028.281528
GTAATAT5700.027.9846081
CTTAGAC5700.027.9846083
Produced by FastQC (version 0.11.5)