FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S17_R1_G12-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S17_R1_G12-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAAAGCCAAAATTGACAAATGGGATCTAATTAAACTCAAGAGTTTCTG29.090909090909092No Hit
GAATTAGAAAAACCACCCTAAAATTTATATGGAACCATAAAACAGCCTGA29.090909090909092No Hit
ACAGGATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACAACCC14.545454545454546No Hit
AATATAGATATGGCAAACATATTTGAGCAGCGCTCCCCTCAAAAACAGGA14.545454545454546No Hit
ACTTACACTTCTCATAGACCAATCAGTTTGCTTTGCTTCAAATTCAGCTA14.545454545454546No Hit
GGGTTCATTTCATGTGGCATATCGAAGTAAGGAAGTTCCTTATCTCCACC14.545454545454546No Hit
GGAGGCATTTGGGGAAGAGTCACATTCTAGAAAAATCTTGGTTTCTGCAG14.545454545454546No Hit
GGTTAAAGGAAAAGAAGTCCCAGGAGAACGGCGAGTCGGGGGCGTGTCAG14.545454545454546No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC14.545454545454546No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG14.545454545454546No Hit
CTTAAATCCTCCCCTTCCCTCCAAACTGCCTGGCCCCTCGTTTGTCTCCC14.545454545454546No Hit
GTACTGAATGAGGAAGCTGAAGTTAAACATAGTGTGTAGGCAGAAGATAA14.545454545454546No Hit
GTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAGATCTACACTCAACT14.545454545454546No Hit
TAAAAACCAATAGTGAATGGTATGAATGAAAATGCAAATTTTCTCCAAAA14.545454545454546No Hit
GGTGGAAGAGACCGACCTCCTGGAGGTGTTGCCGCTCCTCGTCGGTGGTG14.545454545454546No Hit
GGGAAGAGCATACGCCCCTCCTGCTGGAACAGTCGCCTCCGTCTGTCGCA14.545454545454546No Hit
GTGGACATGCCCTTCTCAAAACACTGGATCTTTTCTTTTGAGATGACCGG14.545454545454546No Hit
ATTAGAAATTAAGGTCCTTTAGCCACTCTCTTGCCTAGTGGCTCATTAAA14.545454545454546No Hit
GATTTAAGAGTATCAAGTTTCATAAGAGGAAAGAAAGTGATTCCAAGAGG14.545454545454546No Hit
AAGCGGCACAGATTCAAATGGGTCTCGGGTCATCTGTAGCAGGGATCGGG14.545454545454546No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)