FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S17_R1_E05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S17_R1_E05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544462
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAAGAAGTTGCATAAGTACGATTATAAAAAGTATACCCACTTTTGTC17040.3129695001671375No Hit
CTTCTAAACATTGAAAAAAATTTTTTTAGATTAACTTGTGTGCAGTGTCA16370.30066377451502585No Hit
CTATGTATGTTTAACACAATTACCTCTCAATATATCAATCTCTGCCTACT13330.24482884021290743No Hit
GTATGTGAAGTATATTCTCCAAAAATAAGCACTTTTGACCAAAGTTTATT12570.23087010663737784No Hit
CCCTTGAACATCTCTTCATATATTTGAGCCCTTTTTCATTTCTTTTTCTA12300.22591108286712389No Hit
CTGCAGGACTGGTTGCTAGAACTCAAATCCCGTTAACTTCCTTCTGTGAT12080.22187039683210213No Hit
ATCTAAGGGTATCTTCTAAACATTGAAAAAAATTTTTTTAGATTAACTTG11210.20589132023906168No Hit
ACCTACAGGTCATAACCAGACAGCTCTTACTGTGAGGACCATAATCCACA10840.19909562099834333No Hit
CTTCACATACTATGTATGTTTAACACAATTACCTCTCAATATATCAATCT10580.1943202647751358No Hit
GTGTCAGCATACACCATTTTGTGGACAACCTACAGGTCATAACCAGACAG9940.18256554176416354No Hit
CTTCATATATTTGAGCCCTTTTTCATTTCTTTTTCTAAGAAAAAGGCCTC9910.1820145391230242No Hit
CATACATAGTATGTGAAGTATATTCTCCAAAAATAAGCACTTTTGACCAA9840.1807288662936991No Hit
ATATCAATCTCTGCCTACTTTTCCAACCACTCACAGATGTCTTTCCCTCA9550.17540250742935226No Hit
GCCTCAAGGAGCTGTAGGGTTGTTATGACAAGAATAACAAGAGAACACTC9140.16787213800044815No Hit
GTCCTGTAATGTGGAGCTGGAAATAGAAGAGAGGAAGAATGTGACAGTCC8850.16254577913610133No Hit
TAATACGAGTTCCCTTGAACATCTCTTCATATATTTGAGCCCTTTTTCAT8590.15777042291289384No Hit
TAACTAGACTGTCACAAAGCCGCTTGCCACTGGGTGAGAGAGGCACCAGC8060.1480360429194324No Hit
CAATATATCAATCTCTGCCTACTTTTCCAACCACTCACAGATGTCTTTCC8010.14711770518420017No Hit
GTAAAAATCTGTCCTGTAATGTGGAGCTGGAAATAGAAGAGAGGAAGAAT7820.14362802179031778No Hit
TTACAGGACAGATTTTTACTCTGCTTTTATTTTGCTTTTTCTTCTCATAT7570.13903633311415672No Hit
CTCTAGAATCCTGCCCTCACTGCCAGAGGCAGTGGGAGACCCAGATCCCG7500.13775066028483163No Hit
GTATATAGTCAAGGGCTCGATGGTAATTCCAAACTCGACTCTGGAACTGA7360.13517931462618146No Hit
CTGTTGGGAGTGTTCAGTTTTCTCAACTTTCTGTCATGATCCGCTGAAAG7270.13352630670276347No Hit
AAAGCAGGCTGAGCATGGAGCTTGATGTGAGGCTTGATTTCACAACCCTG7220.13260796896753124No Hit
GAATAACAAGAGAACACTCATTGTTCAATTTTTTATGACTTCTGAAAGAA7170.13168963123229904No Hit
GTCTGTGAGGTTCATGCATTTATCTTCTCTTCCTCCTGAAATGGCACTAA6960.12783261274432375No Hit
TTCTTAAACAGGTCCCCCAAAACTGGCAGAATAAGCACATTACTTAGCAA6670.12250625387997693No Hit
GTCCCATGCTACTTGCCCCGGGTGAGTGCGGCTGACGGATGAGTGCAGCT6620.12158791614474472No Hit
GAATGTGACAGTCCCATGCTACTTGCCCCGGGTGAGTGCGGCTGACGGAT6490.11920023803314098No Hit
GGTCAGTAGGTACTGACTGAACTACCCAGGTGTTAACTAGACTGTCACAA6480.11901657048609454No Hit
CTTTAAATATGAGCACAGTTTCGAAGAGTCAGATCTATCTACAAAACTTA6480.11901657048609454No Hit
GTGTAGGTTGAGGCTTTAAGCCCTCCTGTGCAAGTTTCATTGTCCATTAG6470.11883290293904808No Hit
ATAATAGCCTTGTGTGGTGACAAATGGTAGCCACACTTGCGGTGGGCATA6380.1171798950156301No Hit
GTAATGTGGAGCTGGAAATAGAAGAGAGGAAGAATGTGACAGTCCCATGC6370.11699622746858367No Hit
GTCACATTCTTCCTCTCTTCTATTTCCAGCTCCACATTACAGGACAGATT6330.1162615572803979No Hit
CTTAATGACTGAGCCACCTAGGTGCCTCCATTTGGTAATTTTTAAAAATA6260.11497588445107279No Hit
CTTTGTGCCTGAGCATCAAGGCCTGACATTAATAACAATTTCTGGAAGAC6240.11460854935697992No Hit
ATATAGTCAAGGGCTCGATGGTAATTCCAAACTCGACTCTGGAACTGAAC6230.11442488180993349No Hit
TTATTTACCCAAGTGTGTAGGTCTGCTTTTAGATGGGAAGGGAGATACTG6170.11332287652765483No Hit
GGGTGAGAGAGGCACCAGCATTCTCCTCAGACTGAACTTCTCTCTTCAGG6110.11222087124537618No Hit
ACCTAGGTGCCTCCATTTGGTAATTTTTAAAAATAATCTTTAAATATGAG6080.11166986860423685No Hit
GTTTAACACAATTACCTCTCAATATATCAATCTCTGCCTACTTTTCCAAC6060.11130253351014395No Hit
GATATATTGAGAGGTAATTGTGTTAAACATACATAGTATGTGAAGTATAT5970.10964952558672597No Hit
GCTCTTACTGTGAGGACCATAATCCACATCCTCCACAGTGAACATCTAGT5940.10909852294558664No Hit
GTTTTAATTTGAGTTTATCATAATACGAGTTCCCTTGAACATCTCTTCAT5910.10854752030444732No Hit
GTTTCGAAGAGTCAGATCTATCTACAAAACTTATTTAGGAAATCAGCAGG5910.10854752030444732No Hit
GTGTTAAACATACATAGTATGTGAAGTATATTCTCCAAAAATAAGCACTT5820.10689451238102933No Hit
GTTCTCGGGTAGATTCAGGCCCAGCGTCCCTCTGCAAGCTGCAGGTTCGC5770.10597617464579714No Hit
GATTTTTACTCTGCTTTTATTTTGCTTTTTCTTCTCATATGTTTCTTTCA5720.10505783691056492No Hit
ATGTATGTTTAACACAATTACCTCTCAATATATCAATCTCTGCCTACTTT5660.10395583162828627No Hit
ATCTTGCTAAGTAATGTGCTTATTCTGCCAGTTTTGGGGGACCTGTTTAA5640.10358849653419339No Hit
ATCCCACACTGCAGGACTGGTTGCTAGAACTCAAATCCCGTTAACTTCCT5600.1028538263460076No Hit
CTTGTGTGCAGTGTCAGCATACACCATTTTGTGGACAACCTACAGGTCAT5580.10248649125191474No Hit
GAAGTATATTCTCCAAAAATAAGCACTTTTGACCAAAGTTTATTTGGTCA5580.10248649125191474No Hit
GATTGATATATTGAGAGGTAATTGTGTTAAACATACATAGTATGTGAAGT5580.10248649125191474No Hit
CTCTTACTGTGAGGACCATAATCCACATCCTCCACAGTGAACATCTAGTG5550.10193548861077541No Hit
CTTCTATTTCCAGCTCCACATTACAGGACAGATTTTTACTCTGCTTTTAT5500.1010171508755432No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)