FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S17_R1_D12-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S17_R1_D12-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC18.333333333333332No Hit
GCTCTCTACTGTACTGCGCCTCCGAAGGACATGGACGGGTGGTTGGAGGG18.333333333333332No Hit
ATCCATAATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATAT18.333333333333332No Hit
GCACTCATTCTGAGGGGATCAGTAGCACATAAATCCTGCCTGCCTGCTTG18.333333333333332No Hit
ATCCTGACACTTTGGGAGGCTGAGGCAGGAGGATCACCTTAGCCCAGGAG18.333333333333332No Hit
TAATATTATGACGTTGCAACTAAACATTCATAGATTACCAATATGAATCG18.333333333333332No Hit
GCTTAGAAACATAATGTAGGTAAAGCACTTGGCACAATGTCTAGCACCTA18.333333333333332No Hit
CTCTATTCTTCTGAAAAAGGCCTTTTCTCAGGAAAAACTAGTTAACCACA18.333333333333332No Hit
GCCTTGCCCCTGCTATGGGGCAAGGCTGGATTTATGCAGGCTATATTCCC18.333333333333332No Hit
ATCCGGGCAAGCTCCCGGAAATCGAGATCAGTTGGCTGGCCCCGGGGCAG18.333333333333332No Hit
GCAGGCTTTGAATCAGTCGCTCCACGCGGAATACGAACTGTCTCTTATAC18.333333333333332No Hit
CAATAACTTGGCCTATCAACATAAAAACACAAGTCAATGTGATTGGCCGC18.333333333333332No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)