FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S17_R1_D09-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S17_R1_D09-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences943571
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAACACAATGTAACACAATGCTTGACTTCAGGGGAAATACTCAACCA31010.32864511520595696No Hit
GTGTTACATTGTGTTACATTGGGCCTTACCTCATCTGGTCTTATCAGCAG26710.28307355779268334No Hit
ATGTAACACAATGCTTGACTTCAGGGGAAATACTCAACCAAAAAATCTAG24900.26389111153267747No Hit
GTGTTACATTGGGCCTTACCTCATCTGGTCTTATCAGCAGGGCTGTGAAA22620.2397275880670347No Hit
ATGTAACACAATGTAACACAATGTAACACAATGCTTGACTTCAGGGGAAA22200.2352764126917847No Hit
TCTTATATGTTCTTGATTTTAAGACAAAGGCTGCATGATTTGAGCTAATG21840.23146111951299903No Hit
CTTTAGCGAGTCTCATCGGCTGCAAATCCATAAAACACCCTGCACTTCCA20340.21556406460139194No Hit
TCCCCGAAGTAACTCATTCACCCGTTAAATAAATAGTGATCCAACATCAA20050.21249063398514792No Hit
CCACAGGGCCAGTCCCTGAGTCTGTCACATACACAGCAATACAAGCTTCT15150.16056025460723145No Hit
GAGCAAAAGCAGGGGTAGGGGGCCTGTCAGCTCCATTTCCAGGGCCCCAT14890.1578047650892196No Hit
GAGCAAAAGCAGGCGGAGTAGGAGAGGTAGAAGCAGGCTTCCTGCCAAGC13090.1387282991952911No Hit
ATCCAGGCAGAGTGCACGGGTGTGTCACCGTGAATACTCAGGGAGCCGGA12460.1320515361324161No Hit
GTGTTACATTGTGTTACATTGTGTTACATTGGGCCTTACCTCATCTGGTC11630.12325516574799353No Hit
GTATTAAGGAGGATATCAGTGAGGTTTAAGTTTCAGATGTGTTTTGGGGA10830.11477673646180309No Hit
GTTCTATAGTGTTTGGATAAAAATAAGAAACAGAGAGGAGAGTCCAGCAC10490.11117340401517214No Hit
CTCCCTAAAGGGACAAGATGTTATAAAAAGAAAGAAATACATTGACAGCG10340.10958369852401142No Hit
CTATAGAACAAAAATAAGAAAATCTTATCCATTGCCAGTGGTAGGACTCA10020.10619232680953525No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)