FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S17_R1_C08-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S17_R1_C08-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875342
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATGAGGGGACCTTCATTGAGAATGGCCAATGGGAAATTATCCACAAG18950.21648681315417287No Hit
GTATATAAGGCCATAAGATGTGAGGAGACAAGCTTTCGACAAACCTGTAA17950.20506270691912418No Hit
ATATAAGGCCATAAGATGTGAGGAGACAAGCTTTCGACAAACCTGTAACA17200.19649462724283767No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT14850.1696479775904732No Hit
TCCTAAGGGAGGCGGGGAAGGACAGCGCGAAGAAGTCACCTTCTACCTCA12280.14028802456639805No Hit
GAATAAAGGAAAGCCATCCAGGGCACTGAAGAAGGCACGTCAGGGGAATG11320.1293208825807513No Hit
CCCTAAGCGCACTGAGGGCAGAGCCCACACCTGATTGATCTTCTTGTTCT9340.10670115223535487No Hit
CTCCTTAACTATATAAATTCCAAGGGACATAACTGGGCCACACGGAGTCT9280.10601570586125195No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)