FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S17_R1_B10-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S17_R1_B10-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1075374
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTATATGTTCTTGATTTTAAGACAAAGGCTGCATGATTTGAGCTAATG28800.26781380245384395No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT20380.18951546159754654No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA15010.1395793463483402No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA14130.1313961468289172No Hit
ATGTAACACAATGTAACACAATGTAACACAATGCTTGACTTCAGGGGAAA13020.12107415652600863No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG12650.11763349309170576No Hit
ATGTAACACAATGTAACACAATGCTTGACTTCAGGGGAAATACTCAACCA12570.1168895658626673No Hit
GTCACATACACAGCAATACAAGCTTCTCTACATTTCTAAACATTTTCTGG12170.11316992971747504No Hit
GTATTAAGGAGGATATCAGTGAGGTTTAAGTTTCAGATGTGTTTTGGGGA11650.10833440272872508No Hit
CTTTGGTATGTTACACTTCAGTAAACATTTTACTGAAAATTAAAAAGAAG11460.10656757555975874No Hit
CTATAGAACAAAAATAAGAAAATCTTATCCATTGCCAGTGGTAGGACTCA11390.1059166392343501No Hit
GAGCAAAAGCAGGGGTAGGGGGCCTGTCAGCTCCATTTCCAGGGCCCCAT10780.10024419411293188No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)