FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S17_R1_B08-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S17_R1_B08-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980391
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATACCGTACACAAAATTAAAAATGGATTAAGGACCTAGGGGCACC44180.45063653175110746No Hit
GTATTACACGTCACGATAAACGTGAATTGGGTGTGTTAACCGGGAAGAGG36300.3702604369073156No Hit
GTTCTTAGGTGTGACAGAAGAAAATTAATGAACGTGCCCTTCCACATTTG24560.2505122956045088No Hit
CCTCTAGGGTGCGCCTGTCCCTGAGTGCCAAGACTTATGAGCTAGATGGC23780.24255628621641773No Hit
GGATAAAGGAGATGTCCATTATTTGTTCTCTCCACCATCCAGTGAAGCAT22880.23337627538400496No Hit
GCTATATACAAAAGAATGAAACCGGACCAGTGTCTTATACCGTACACAAA21680.2211362609407879No Hit
CCTAAGAACTGGTGATGGGTAGTTTGGTTTCTGCCAGGGTCAGAGAGAGT20600.2101202479418926No Hit
ACCCTATGAGATACACATAATATGATCCCCATTTTGCAGTTAAGGAAACA19280.1966562320543538No Hit
TCCCTGAACCATGAGGGCATGGGAAGGCTGCCATAGCTCTGGCTCCACTC19270.19655423193399366No Hit
TCATAGGGTTGTGTGAGACTGAGGCAGTCAACATGTGTTAGGTAAGCACT19120.19502423012859155No Hit
GAATAAGATGGAGGGCCAAAGATGGAGTCAGTGTTTCAGAACTCCTAGCC19080.19461622964715097No Hit
GAAGAATATAGAGCCCAGAAATAAACCTGCAATTATATGGTCAATTAATC18590.18961822374950402No Hit
GTTCAATGGCTGAGCCAAGCCTTGAATGGCATGTCAGGTAGGCCAGGAAA18440.18808822194410188No Hit
CCCTTAGACACTCTCCCCATCTCTAGGTCCTTCCTTATCTCTCCCTGGAG17380.17727620918592685No Hit
GTATAAAGACCTTTAAAAAAAAAAATGTTACATTAGTCACCACACAGTAC17230.1757462073805247No Hit
GTCTAGGTACAGTCAACAGGATTTGCCCATCACGTGTGGGGTGAGAAAGA16710.17044220112179734No Hit
GGTAAGCACTGCGTGCATGTGGCTTAGTCCAGGAGGTAGGTAAGTAGTAG16230.1655461953445105No Hit
GTCTGGGGCGGTCTGTGAGCAGGACCAGCTGGGCATGGAATGGGGCCCAC15980.16299619233550697No Hit
GTTCAAAGGCGCGCAAAGTAGAGCAGTACTGTGTTCCCATTCGGTACCCC15340.15646818463245787No Hit
GTGCAGGTGGGTGTTCTTGTTCACGGTCAGGCTCCTGGCTGGACTCAGCA15190.15493818282705574No Hit
TCTCCGCACTTACTTCAAGGAGAACCAGGAGGTCCGGGAGTGGGATTTCC15190.15493818282705574No Hit
GTGGAGGAGATCCAAGGAAGGACAGTCACAGAATCAGGGAACGTCAGTTT14490.14779817440184578No Hit
CTTTTAATGATGGGCGATCCAGGAAGGACTTCTTGAAGTTAACTTTTGAG14280.1456561718742828No Hit
GTATTTATACATATTTCAGGGGAGGAGAATGGAAGCATGGGTGAGCCTGG13950.14229016790239812No Hit
CTCTAGGTCCTTCCTTATCTCTCCCTGGAGAAATCCTTCTCCAGTTTCAA13830.14106616645807643No Hit
GTTCAGGGAATGGCCCTTGGTCATCCAAATGTGGAAGGGCACGTTCATTA13830.14106616645807643No Hit
TGTCAGATGAAGTCCCATGTTCAAAGGCGCGCAAAGTAGAGCAGTACTGT13710.13984216501375474No Hit
AAGTAGAGCAGTACTGTGTTCCCATTCGGTACCCCGAAGAACCCCGAAGA13560.1383121632083526No Hit
GGCCTATACACCCCTAGGCTGGAGTTGCCATCAGTGAGAAGGGGAAGTAC13400.1366801612825903No Hit
GTACTGTGTTCCCATTCGGTACCCCGAAGAACCCCGAAGAACCCCGCGGT13300.1356601600789889No Hit
TATTATGTGTATCTCATAGGGTTGTGTGAGACTGAGGCAGTCAACATGTG13130.13392615803286648No Hit
GCCTTGAATGGCATGTCAGGTAGGCCAGGAAACGAGGTGAACAAAATCAC13130.13392615803286648No Hit
GTCACAAGGATATCTGACAGAAGGGCACTCCTGAAAGATGGAACAGCAAG13120.13382415791250635No Hit
GTATAGGCCAGGAACCCAGGAGTCTCCAGCCTTCAGCCTTCTTTCTCACC13050.13311015706998533No Hit
GTCCAGGAGGTAGGTAAGTAGTAGTTGGATATGGGACGTGTTGTCTAGGT12970.1322941561071042No Hit
GCCCCAGACAGGTCCCCAGGGCAGGGCCCGGAAAGCAGGGCATCCTGGGT12930.13188615562566364No Hit
CCTTAATGCTGCTTCTTGGCTACACATGGTCCCCATCAAAGTGACCCCAT12910.13168215538494335No Hit
TTTCAGGACAGAAGGGAGCATCTCCGCACTTACTTCAAGGAGAACCAGGA12820.13076415430170207No Hit
CATCAAAGGAGGCAAGAATATGCAATGGGGGGAAAAAAGTCTCTCCAACA12580.1283161514130587No Hit
ACCAAGGGCCATTCCCTGAACCATGAGGGCATGGGAAGGCTGCCATAGCT12530.12780615081125796No Hit
GTGCTTACCTAACACATGTTGACTGCCTCAGTCTCACACAACCCTATGAG12500.1275001504501775No Hit
TGCTTGTACATTCCAGGGTTACAAGGAGCTTGGGGCTGGAGCCAGAGGGA12300.1254601480429747No Hit
CCCCTAGGCTGGAGTTGCCATCAGTGAGAAGGGGAAGTACATGGGGGTTA12240.12484814732081383No Hit
GAAGGGAGCATCTCCGCACTTACTTCAAGGAGAACCAGGAGGTCCGGGAG12100.12342014563577185No Hit
CCCATGCATCCTGCAATTTGTTAGTTTTGCTGTTGCTTAGCAAGACATTC12070.12311414527469142No Hit
GTACATGGGGGTTAGGGAGCAGGTCTGGTTAGTAGAGGAATTTAGACTCA12040.122808144913611No Hit
ATCCTGGGTCATCCGACCATTCTGGATCAGAAACCGTGGGGCATGGTCTG12020.12260414467289071No Hit
GTGTGAGACTGAGGCAGTCAACATGTGTTAGGTAAGCACTGCGTGCATGT11990.1222981443118103No Hit
CTCTGGGCCTGTTTCCTTAACTGCAAAATGGGGATCATATTATGTGTATC11850.1208701426267683No Hit
GTTCTGCTCCTTCCCTCTGTGGGCCCCATTCCATGCCCAGCTGGTCCTGC11780.12015614178424731No Hit
CATTTAAGGTGTGCTTCATGTAAGATCTAGCTTCTGGCTTTCTCCCTTAG11740.11974814130280673No Hit
GAATTAGGGGGCTCTCTTTCCTCTTCCCGGTTAACACACCCAATTCACGT11680.1191361405806459No Hit
ATCTATAACAAATACATCAAAGGAGGCAAGAATATGCAATGGGGGGAAAA11680.1191361405806459No Hit
GTAATAAGGAGAACACATTTTGCAACGAGCACTGGATGTTATATGCAAAC11640.11872814009920532No Hit
GCCTACTTGTGATCTCTGTCTGTCAAATAAATAAATAAAATCTTAAAAAA11520.1175041386548836No Hit
GTCTCACACAACCCTATGAGATACACATAATATGATCCCCATTTTGCAGT11360.11587213672912135No Hit
CTCTGAGGGTCCGCAGCACCATCTCGAGTCTGTGGTCATATCCACAAGAA11260.11485213552551991No Hit
CTCCTGAAAGATGGAACAGCAAGTGCAAAGGCCCCGGAGCTGTACTCCTG11180.11403613456263878No Hit
CTCTCTGACCCTGGCAGAAACCAAACTACCCATCACCAGTTCTTAGGTGT11180.11403613456263878No Hit
CATAATATGATCCCCATTTTGCAGTTAAGGAAACAGGCCCAGAGTGGTCA11070.11291413323867722No Hit
ATCCAGGAAGGACTTCTTGAAGTTAACTTTTGAGCAGAGCCCTGAAGGAG10980.11199613215543594No Hit
ATTAAGATGTGAGCAGAAGACTGATGTGACATGACCCATGGGAACTAAGT10920.1113841314332751No Hit
GTGGGAGGGAGGCCTGAAGGGCAGAAACTCGGGGACAACTTTCTCTGCGG10870.11087413083147439No Hit
ACATTGGGCTCTCTGCTCAGCAGGGAGCCTTCTTCCTCCTCTCTCTCTCT10670.10883412842427154No Hit
CTTCAAGGAGAACCAGGAGGTCCGGGAGTGGGATTTCCAGTCTTCTTTGG10530.10740612673922954No Hit
GGACAGAAGGGAGCATCTCCGCACTTACTTCAAGGAGAACCAGGAGGTCC10060.10261212108230289No Hit
GGCCCAGAGTGGTCACATGGCTCGTAACTGGTCCCCTGCTTTCCTCTTTA10060.10261212108230289No Hit
GTCCTGGGATCGAGCCCCACATTGGGCTCTCTGCTCAGCAGGGAGCCTTC9970.1016941199990616No Hit
ACCCAGGATGCCCTGCTTTCCGGGCCCTGCCCTGGGGACCTGTCTGGGGC9920.1011841193972609No Hit
GGCTGGTATCCCACCCAGGTCGCCATGCTGGGCAGGTTGGAGCCCAAGTG9890.10087811903618046No Hit
GTTACAAGGAGCTTGGGGCTGGAGCCAGAGGGAGTGAGGCCTGGAATGAG9860.10057211867510003No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)