FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S17_R1_A12-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S17_R1_A12-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023209
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGT45790.44751365556792405No Hit
GTATTTGTTATTGGTCCATTCCTATTCCACCATGTCACAGCCAGAGGTGA39740.3883859504754161No Hit
GGATGTAATCATGGAAGTTGTTTTCCCTAACGAAGTGGGAGCCAGGATAC32560.31821455831604295No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG31010.30306613800308635No Hit
CTTCATATCCCTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTA30380.2969090381339492No Hit
ATGTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAA29430.2876245224582661No Hit
GTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATT28450.27804681155071936No Hit
GATGTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCT27110.26495075786080846No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT26090.2549821199774435No Hit
GCCTAAGGATGTCTACCATCCTAATTCCACCAATCTGTGTGCTGTGGCAC25960.25371160730603426No Hit
GATATATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTT25950.2536138755620797No Hit
GTTTTATTATCTGTGCGGTATCAAATGTCCCAAGCACATCCCTCATTTGT25740.25156150893903395No Hit
ATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAA23970.2342629902590771No Hit
GAACAGAGAAACTGACAATAACTTACTCATCGTCAATGATGTGGGAGATT23130.22605352376689417No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG23060.22536940155921223No Hit
TTATTGTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTC22900.2238056936559393No Hit
GCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTG21960.21461890972421077No Hit
ATCCCAATCATTCCCATCACATTGTCGATAGGTTCAACTCCCCAATTTTG21820.21325066530884698No Hit
AACTTACTCATCGTCAATGATGTGGGAGATTAATGGTCCTGAATCAGTGT21380.2089504685748464No Hit
GTGTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAG21290.20807088287925538No Hit
CTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATTCCATTTTATT21200.20719129718366433No Hit
GTATCAAATGTCCCAAGCACATCCCTCATTTGTTGGAACAGAGTTCTTAC21090.20611624800016418No Hit
GGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTC20550.20083873382661802No Hit
CTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTC19660.1921406086146623No Hit
GTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAACT19640.19194514512675317No Hit
GTCGATAGGTTCAACTCCCCAATTTTGAAAAAGCACTTTCGCATCCTTCT19510.1906746324553439No Hit
GATATGAAGAGTTCACAATGGTTGGGAGAAGAGCAACAGCCATACTCAGA19080.1864721674652979No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC19080.1864721674652979No Hit
GTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGAT19070.18637443572134335No Hit
GTGTTGGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAA18560.18139011677966085No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT18560.18139011677966085No Hit
GATAAAAGCAGTCAGAGGTGATCTGAATTTCGTCAATAGGGCGAATCAGC17780.17376704075120528No Hit
CTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTCTT17660.1725942598237506No Hit
GTATTTTCACAAGAGGATTGTATGATAAAAGCAGTCAGAGGTGATCTGAA17630.1723010645918869No Hit
TTGCAATAGTGTGTTGGAATTTTTTGTGTCTCTCACTCGGAAGGACATAT17510.1711282836644322No Hit
GTGCTGGAGTACTCATCTACACCCATTTTGCTGATTCTCACTCCTCTCAT17420.17024869796884115No Hit
GCATATATCCACGGCTTGCTCTTCTGTTGGGTTCTGCCTAAGGATGTCTA17070.1668280869304316No Hit
CTACAATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTA16820.16438479333156764No Hit
GTCTGATCCTGCATCATTCATTTTACTCCATAAAGTTTGTCCTTGCTCAT16400.16028006008547618No Hit
ATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTCAGTGG16370.15998686485361252No Hit
CAATAATTGTGGCCATGGTATTTTCACAAGAGGATTGTATGATAAAAGCA16130.1576413029987031No Hit
CTCCAACATGTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTT15760.15402522847238442No Hit
CTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCTTGATTATT15690.15334110626470251No Hit
GTGAAACACAGGGAACAGAGAAACTGACAATAACTTACTCATCGTCAATG15670.1531456427767934No Hit
CAATTATTGCTTCGGCAATCGACTGTTCGTCTCTCCCACTCACTATCAGC15280.14933410476256562No Hit
CCACTACCCTCTCCGTGCTGGAGTACTCATCTACACCCATTTTGCTGATT15260.14913864127465648No Hit
GAAGATAAGAGTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAGAA15170.14825905557906546No Hit
CATCTACACCCATTTTGCTGATTCTCACTCCTCTCATTGACATCTCGATG15160.1481613238351109No Hit
GACAATAACTTACTCATCGTCAATGATGTGGGAGATTAATGGTCCTGAAT15030.14689081116370165No Hit
CTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAATATCCCAATCAT14970.1463044206999743No Hit
GGATACTAACATCGGAATCGCAACTAACGATAACCAAAGAGAAGAAAGAA14920.14581576198020152No Hit
GGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCCGGGACCAACGAGGA14900.14562029849229238No Hit
GTAGTACATTTCCTCGTTGGTCCCGGATTCTCAAAAAACGGTCAATGCTC14870.1453271032604287No Hit
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC14760.14425205407692857No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA14710.14376339535715577No Hit
AGATAAGAGTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAGAAGA14450.14122237001433724No Hit
GTTTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCC14430.14102690652642813No Hit
GATTAATGGTCCTGAATCAGTGTTGGTCAATACCTATCAATGGATCATCA14370.14044051606270078No Hit
GTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTCAAT14330.14004958908688253No Hit
ATTACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGACCAGGATTTAT14230.13907227164733696No Hit
CTATCAGCTGAATCAATCTCCTGGTTGCTTTTCTGAGTATGGCTGTTGCT14140.13819268595174594No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT14040.13721536851220034No Hit
GTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTGATGATCCATT13970.13653124630451843No Hit
GTACTAAAGACTGAAATGGTTCAAATTCCATTTTATTGTATAGCATTGTA13730.13418568444960902No Hit
GTTGAATACAGGAGAATTGCCCCTTACAAGTATTCTCATTCCTGATCCCC13660.13350156224192714No Hit
GTATTGACCAACACTGATTCAGGACCATTAATCTCCCACATCATTGACGA13610.1330129035221543No Hit
GCTTTTATCATACAATCCTCTTGTGAAAATACCATGGCCACAATTATTGC13530.13223104957051784No Hit
CTCTTGACTGATGATCCGCTTGTTCTCTTAAATGTGAATCCACCAAAACT13270.12969002422769932No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA13150.1285172433002446No Hit
CTTCTGAAAATGTCTTAAAAGTTGATGCATAGGATTCAATCGCTGATTCG12970.12675807190906258No Hit
AACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGAG12900.12607394970138064No Hit
TTCTCACTCCTCTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAAT12760.12470570528601682No Hit
GAATTAGCTCATCCTTCAGTTTTGGTGGATTCACATTTAAGAGAACAAGC12680.12392385133438036No Hit
CCATAAAGTTTGTCCTTGCTCATTTCTCTCAGGAATCATTTCCGTTATCC12530.12245787517506199No Hit
GAATCAGTGTTGGTCAATACCTATCAATGGATCATCAGAAACTGGGAAAC12520.12236014343110742No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT12490.12206694819924374No Hit
ATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAAGGCCA12440.12157828947947096No Hit
CTTTAGTACCTAAGGCCATTAGAGGCCAATACAGTGGGTTTGTAAGAACT12340.12060097203992538No Hit
CATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATG12300.12021004506410714No Hit
GTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGATGT12290.12011231332015258No Hit
ATTGAATCCTATGCATCAACTTTTAAGACATTTTCAGAAGGATGCGAAAG12150.11874406890478877No Hit
GGCTAAAGCATGGAACCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAA12150.11874406890478877No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC12090.11815767844106141No Hit
TCCTAATTCCACCAATCTGTGTGCTGTGGCACATCTCCAATAAAGATGCT12050.11776675146524317No Hit
GGATTCAATCGCTGATTCGCCCTATTGACGAAATTCAGATCACCTCTGAC11940.11669170228174303No Hit
GTAAATGAGGAGAACTGCATTCTACTTTGCTTTGGTGGAGCGGCTGCGAA11900.1163007753059248No Hit
GTCCATTTTAGAAACCAAGTCAAAATACGTCGGAGAGTTGACATAAATCC11870.11600758007406112No Hit
TGTTATAGTTGAATACAGGAGAATTGCCCCTTACAAGTATTCTCATTCCT11820.11551892135428833No Hit
GTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCTTGA11800.1153234578663792No Hit
GTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTTCTTCTTTCT11790.11522572612242465No Hit
GCAATAGTGTGTTGGAATTTTTTGTGTCTCTCACTCGGAAGGACATATGG11720.11454160391474273No Hit
TAATAAAACTTCTTCCCTTCGCAGCCGCTCCACCAAAGCAAAGTAGAATG11600.11336882298728804No Hit
CTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTT11420.11160965159610597No Hit
TCAATGATGTGGGAGATTAATGGTCCTGAATCAGTGTTGGTCAATACCTA11400.11141418810819687No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG11340.1108277976444695No Hit
ATCCTGCATCATTCATTTTACTCCATAAAGTTTGTCCTTGCTCATTTCTC11320.11063233415656039No Hit
ATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTCAGTGGTCCCAG11250.10994821194887847No Hit
TTTCAGAAGGATGCGAAAGTGCTTTTTCAAAATTGGGGAGTTGAACCTAT11180.10926408974119657No Hit
TTCCTGATCCCCTCACATTCACAGTAAATGAGGAGAACTGCATTCTACTT11150.1089708945093329No Hit
ACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGATGTAT10840.10594121044674157No Hit
TTCCTATTCCACCATGTCACAGCCAGAGGTGATACCATCACTCGGTCTGA10780.10535481998301423No Hit
GTACTCATCTACACCCATTTTGCTGATTCTCACTCCTCTCATTGACATCT10780.10535481998301423No Hit
GAACAGATGTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCA10760.10515935649510509No Hit
ATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTTTGGTG10680.10437750254346863No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG10650.10408430731160495No Hit
GATCCTGCATCATTCATTTTACTCCATAAAGTTTGTCCTTGCTCATTTCT10530.10291152638415026No Hit
TCTGTGTGCTGTGGCACATCTCCAATAAAGATGCTAGTGGATCTGCTGAT10530.10291152638415026No Hit
TCTCAGTGCCAAGGAGGCACAGGATGTAATCATGGAAGTTGTTTTCCCTA10490.10252059940833201No Hit
GTCCTGAATCAGTGTTGGTCAATACCTATCAATGGATCATCAGAAACTGG10330.10095689150505908No Hit
GGCCAATACAGTGGGTTTGTAAGAACTCTGTTCCAACAAATGAGGGATGT10280.1004682327852863No Hit
CCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAAATACGTCGGAGAGTT10250.10017503755342261No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)