FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S17_R1_A06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S17_R1_A06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830892
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT35000.42123404726486713No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT13350.1606707008853136No Hit
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA11220.1350356002946231No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA10420.1254073934999976No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG10310.12408351506573657No Hit
GTATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGA9560.11505707119577516No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA9270.11156684623272338No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA8910.1072341531751419No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC8340.1003740558339712No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG8320.10013335066410556No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)