FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S16_R1_G03-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S16_R1_G03-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819655
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAGTAAAGTGGCTCACCCTACAGCTGCCTCGGGAAGTGGAGATGGTG20450.24949521444998202No Hit
CTCTTAATGTATCACCCAAGTTGGGTTCAAAAATAAGGAAGATGCAACTC19450.23729495946465282No Hit
GATATAGAGTGTGGGTTTTAGAGTTAGTGAGATCTCAATCCAATCCTGTC17120.2088683653488358No Hit
TAATTATACACTGCAACCCAATTCTATGACCTGATCCTTTCTTTTTCATA16210.1977661333121862No Hit
GTGTTAGCCATCTTTATTGTCATAATTATACACTGCAACCCAATTCTATG15990.1950820772154138No Hit
GTCCAGGATATTTAAAATGCAGGGAATGACCAAATGTGTTTAAACGCTGT15900.19398405426673418No Hit
CCCTACAGCTGCCTCGGGAAGTGGAGATGGTGGGGCAATGTGGCTGGTGC15460.18861594207318932No Hit
GATAAATACTGTCTTTACGAAGTCTATGTTTCTTCATCTGTAAAATGGGG14930.18214980693096486No Hit
ATTATACACTGCAACCCAATTCTATGACCTGATCCTTTCTTTTTCATATG13980.17055956469490213No Hit
GTATAATTATGACAATAAAGATGGCTAACACAATGCAAAAACCTTTATTT13890.1694615417462225No Hit
TGATAATACCTCCTTGGAATTGTTAGGAGGATAAAGAGAGATAGTAAAAT13480.16445943720223752No Hit
CTATAGAATACTTCATATATGCGAGCTCTTAATGTATCACCCAAGTTGGG13190.16092136325649206No Hit
CCTTATATTTGAGATATGGATAATGATAATACCTCCTTGGAATTGTTAGG12980.15835930970957293No Hit
ATCTTACACTAGGACAGATACCTGTTTTCAAGGAAATCTTCTAGTTTGGG12880.15713928421104No Hit
ATATAATATCTCTCACATGCCTAGCACCCAGCAGGTTCTTAGGATTTGAA11850.14457302157615096No Hit
GGATAAAGAAAAGGATACGCAAAAGAAGCGAACGGAAATTTCAGAGATAA11830.14432901647644436No Hit
ATCCTGGACCATCCCCAGGATACCAGTGCACTGCTAAGCCTCATGGGAGA10610.12944470539434275No Hit
GTGTATAATTATGACAATAAAGATGGCTAACACAATGCAAAAACCTTTAT10530.12846868499551642No Hit
TTATAACACAGGTAATAAATGCTACTACCATTGAGTTAAGTCCAGAATGT10100.12322257535182485No Hit
GGCATATAATATCTCTCACATGCCTAGCACCCAGCAGGTTCTTAGGATTT10030.1223685575028518No Hit
GTTTAAAAACTATAGAATACTTCATATATGCGAGCTCTTAATGTATCACC10000.12200254985329194No Hit
GGCTAACACAATGCAAAAACCTTTATTTTACTATCTCTCTTTATCCTCCT9660.11785446315828No Hit
TCTTTATCCAGTGTGTTGAAGTTCTACCTCAGACCCTCACGAACATTTGT9480.11565841726092076No Hit
GGTAATAAATGCTACTACCATTGAGTTAAGTCCAGAATGTTATGAGAGCC9400.11468239686209443No Hit
GTATTAAATGGTAGCCTAACTGGCCTGTTGACTCTTTTTGTCTTTACATC9360.11419438666268125No Hit
ATTTAATACACACAGAATTTTTAGAGTAGATATTGGAAGATTAGATGAGA9320.11370637646326807No Hit
CTCTTTATCCTCCTAACAATTCCAAGGAGGTATTATCATTATCCATATCT9230.11260835351458846No Hit
GATAAAGAGAGATAGTAAAATAAAGGTTTTTGCATTGTGTTAGCCATCTT9220.11248635096473517No Hit
GTATTTATCTGGTGGGTGGTAGATTTGAATGTATGTGGTATGGTTCCAAA9210.11236434841488188No Hit
GCCTAGCACCCAGCAGGTTCTTAGGATTTGAAGAAGAGGCAACTATAAAC8850.10797225662016335No Hit
GTATTGCAGGAATGGAAGAATGTGACTGGAATGGTAGATTGGGTCCTGGT8840.10785025407031007No Hit
ATATATGCGAGCTCTTAATGTATCACCCAAGTTGGGTTCAAAAATAAGGA8620.10516619797353766No Hit
CTTCATATATGCGAGCTCTTAATGTATCACCCAAGTTGGGTTCAAAAATA8560.1044341826744179No Hit
GTGTATGACCTAGGTCAAATTATTTTTCTGTGCCCTAGCCTCCTTATATT8550.1043121801245646No Hit
CATTAAGAGCTCGCATATATGAAGTATTCTATAGTTTTTAAACAGCGTTT8480.10345816227559156No Hit
TCATATATGCGAGCTCTTAATGTATCACCCAAGTTGGGTTCAAAAATAAG8290.10114011382837901No Hit
TCTATGACCTGATCCTTTCTTTTTCATATGGCCAATTTTGTTGAGTTGCA8240.10053010107911255No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)