FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S16_R1_F05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S16_R1_F05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992751
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGGCGTCTCCATCCTGAATCTTGGACAAAAGAGATACACCAAGAC84510.8512708624821329No Hit
CTATTAAGAGCGGTCGGATTTTTTCAATCTTCTTTCTTGTTGAATCATTG67370.6786193113882535No Hit
CTTCTATTGTGTTGGCCAATGCTGTTGCAGCAGGTTGGTTTCTATTTAGA39660.3994959461133759No Hit
TTCCTGAAGTCTGCCTAAAATGGGAATTGATGGATGAGGATTACCAGGGG38660.3894229267963467No Hit
GAGTATATGTGAGAAACTTGAACAATCAGGGTTGCCAGTTGGAGGCAATG36990.37260098453690804No Hit
CTCTTAATAGAGGGGACTGCATCATTGAGCCCTGGAATGATGATGGGCAT34910.3516491043574874No Hit
GCCTAATACAGTGCTTAACATATTGAACATGCCCATCATCATTCCAGGGC34610.3486271985623787No Hit
CTCCAATACTCATGTCCGCTGACTCGTTGATCCCAGACACCCCAAAACTG33380.3362373848024328No Hit
CATATATGACAAGAAATCAGCCCGAATGGTTCAGAAATGTTCTAAGTATT32560.32797750896246897No Hit
CAATAGAAGTGTTCAGATCAAATGGCCTCACGGCCAATGAGTCTGGAAGG31930.3216315067927406No Hit
TTTCTGAACCATTCGGGCTGATTTCTTGTCATATATGTGATCATGGCCAA31580.3181059500317804No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA31540.3177030292590992No Hit
GTATACAAACCCCCTTATTTGCATCCCTGGGGTTGCAATTGCTCTCCGTT29900.3011832775791714No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT29570.2978591812045518No Hit
GTATTGGAGTTACTGTCATCAAAAACAATATGATAAACAATGATCTTGGT29480.29695260946601915No Hit
CATATACTCCTTGCCAGTGTCTCAACAAAGTATACAAACCCCCTTATTTG28870.2908080676826314No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG28640.2884912732397147No Hit
CATCAGTACTCAGAAAAGGGAAGATGGACAACAAACACCGAAACTGGAGC28070.28274965222900805No Hit
GTTCAGAAATGTTCTAAGTATTGCTCCAATAATGTTCTCAAACAAAATGG26400.26592770996956944No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG26360.2655247891968882No Hit
ATATTGAACATGCCCATCATCATTCCAGGGCTCAATGATGCAGTCCCCTC26310.2650211382310368No Hit
ACTTAGAACATTTCTGAACCATTCGGGCTGATTTCTTGTCATATATGTGA25800.25988389837935194No Hit
CCTCTATGGCATCGGTACGTGTACCTGTAATCTTTGATGAACAACTGAAG25620.2580707549022867No Hit
GTATATGTGAGAAACTTGAACAATCAGGGTTGCCAGTTGGAGGCAATGAG24490.24668824307404374No Hit
ATTAAGAGCGGTCGGATTTTTTCAATCTTCTTTCTTGTTGAATCATTGAA24410.2458824015286814No Hit
CTTCAGGAATGTGGAGATTTCTAATGTTGTATAAATTTGGGCCTCCGTCG24080.2425583051540618No Hit
AATTAGAGCATTGACCCTGAACACAATGACCAAAGATGCTGAGAGAGGGA24070.2424575749608915No Hit
GTTTGTATACTTTGTTGAGACACTGGCAAGGAGTATATGTGAGAAACTTG24050.2422561145745509No Hit
TCATATATGTGATCATGGCCAAAAACATCCGAGGATTCTGATTTTCGTTC23680.23852909742725012No Hit
TCCTTAAGGAAGTCTATGAGCCTTCCAGACTCATTGGCCGTGAGGCCATT23370.2354064614389711No Hit
ATGTTAAGCACTGTATTAGGCGTCTCCATCCTGAATCTTGGACAAAAGAG23300.23470135008677906No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT23240.2340969689277573No Hit
GTCATAGGTCTGTCGGCCTTGTGTCAGCTTGTCTACTCGTGTTTGCTGAA22810.2297655706214348No Hit
GTTCTAAGTATTGCTCCAATAATGTTCTCAAACAAAATGGCGAGACTGGG22610.22775096675802897No Hit
CATATACCCTTTTCCCAGTCTCGCCATTTTGTTTGAGAACATTATTGGAG22330.22493052134926078No Hit
GTTCAATATGTTAAGCACTGTATTAGGCGTCTCCATCCTGAATCTTGGAC22120.22281518729268465No Hit
GGCAAGGAGTATATGTGAGAAACTTGAACAATCAGGGTTGCCAGTTGGAG21790.21949109091806504No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT21640.2179801380205107No Hit
ACATTAGAAATCTCCACATTCCTGAAGTCTGCCTAAAATGGGAATTGATG21120.21274216797565554No Hit
CCCCAAAACTGGGGAGCTCCATGCTGAAATTGGCAACAAACCCATAACGA21080.21233924720297434No Hit
GTATAAATTTGGGCCTCCGTCGGAGACCAGCAGTCCAGCTTTGGAACGGG20950.21102975469176055No Hit
GATTGTGAATGCACCCAATCATGAAGGGATTCAAGCCGGAGTCGACAGGT20480.2062954356127569No Hit
GTCATATTGTCTCTCACCCGTCTCTTTCTCTGAAAATGAGTTGTGATCCC20240.20387791097666988No Hit
TTCACAATCAGAGCAAAATCGTCAGAGGATTGAAGACCATCCCACCAGTA20110.2025684184654561No Hit
TTCTAATGTTGTATAAATTTGGGCCTCCGTCGGAGACCAGCAGTCCAGCT19590.19733044842060093No Hit
GTATTTGTGTGTCACCTCTATGGCATCGGTACGTGTACCTGTAATCTTTG19500.1964238766820683No Hit
GTGTATTGGAAGCAATGGCTTTCCTTGAGGAATCCCATCCTGGTATTTTT18680.1881640008421044No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT18480.1861493969786986No Hit
TAATAGAGGGGACTGCATCATTGAGCCCTGGAATGATGATGGGCATGTTC18300.18433625350163335No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG18140.18272457041090867No Hit
GTTAAGCACTGTATTAGGCGTCTCCATCCTGAATCTTGGACAAAAGAGAT18060.18191872886554633No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT17850.17980339480897023No Hit
CCATAGAGGTGACACACAAATACAAACCCGAAGATCATTTGAAATAAAGA17840.17970266461579992No Hit
TCCTTAAGGATGTAATGGAGTCAATGAACAAAGAAGAAATGGGGATCACA17760.1788968230704376No Hit
TTATTGGAGCAATACTTAGAACATTTCTGAACCATTCGGGCTGATTTCTT17690.17819171171824555No Hit
AATTTATACAACATTAGAAATCTCCACATTCCTGAAGTCTGCCTAAAATG17460.17587491727532886No Hit
TCACTGCATTGTTCATTGATTCAATTTCTTTATGGCTGACAAATGGGTTC17380.17506907572996652No Hit
TCACTGGAGATAACACCAAATGGAACGAAAATCAGAATCCTCGGATGTTT17360.17486761534362594No Hit
ATCCAGGAGTGTGTTGTTGCAACAGCATCATACTCCATGTTTTTGGCTGG17300.1742632341846042No Hit
CCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGG17160.17285301148022011No Hit
TCCTTGAGGAATCCCATCCTGGTATTTTTGAAAACTCGTGTATTGAAACG17150.17275228128704984No Hit
TTTCTAATGTTGTATAAATTTGGGCCTCCGTCGGAGACCAGCAGTCCAGC17140.17265155109387953No Hit
GTATGAACTGCTGGGGAAGAATTTTTCAAATAAATTGCAGCACCTTTGGT17130.17255082090070925No Hit
GCTTAACATATTGAACATGCCCATCATCATTCCAGGGCTCAATGATGCAG17060.1718457095485172No Hit
GGGTATATGTTTGAGAGCAAGAGTATGAAACTTAGAACTCAAATACCTGC17030.17154351896900633No Hit
CCTCTATTAAGAGCGGTCGGATTTTTTCAATCTTCTTTCTTGTTGAATCA17010.17134205858266574No Hit
ACCTATGACTGGACTCTAAATAGAAACCAACCTGCTGCAACAGCATTGGC16540.16660773950366206No Hit
CATTATTGGAGCAATACTTAGAACATTTCTGAACCATTCGGGCTGATTTC16450.16570116776512941No Hit
CAATGATGCAGTCCCCTCTATTAAGAGCGGTCGGATTTTTTCAATCTTCT16210.16328364312904242No Hit
TACATAAACAGAACAGGTACATTTGAATTCACAAGTTTTTTCTATCGTTA16090.16207488081099894No Hit
CACCAAGACTACTTACTGGTGGGATGGTCTTCAATCCTCTGACGATTTTG16030.16147049965197718No Hit
CTATTGTGTTGGCCAATGCTGTTGCAGCAGGTTGGTTTCTATTTAGAGTC16000.16116830907246632No Hit
GTGTTATCATTTTCTTAGTCATATTGTCTCTCACCCGTCTCTTTCTCTGA15890.16006027694759312No Hit
GTGCTTAACATATTGAACATGCCCATCATCATTCCAGGGCTCAATGATGC15640.15754202211833582No Hit
CAATATGACTAAGAAAATGATAACACAGAGAACAATAGGTAAAAAGAAGC15410.15522522767541913No Hit
GTCTATGAGCCTTCCAGACTCATTGGCCGTGAGGCCATTTGATCTGAACA15380.15492303709590824No Hit
CTCCTTGCCAGTGTCTCAACAAAGTATACAAACCCCCTTATTTGCATCCC15370.15482230690273796No Hit
GTCTTGGTGTATCTCTTTTGTCCAAGATTCAGGATGGAGACGCCTAATAC15360.15472157670956765No Hit
GAACAGGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAA15260.15371427477786473No Hit
GCTCTAATTAGATAACTCCTTTTGTTCAATCTCTGCTTCTTTTTACCTAT15150.15260624265299155No Hit
CCTTAAGGATGTAATGGAGTCAATGAACAAAGAAGAAATGGGGATCACAA15150.15260624265299155No Hit
ATCATGAAGGGATTCAAGCCGGAGTCGACAGGTTTTATCGAACCTGTAAG15060.1516996709144589No Hit
CAGTAACTCCAATACTCATGTCCGCTGACTCGTTGATCCCAGACACCCCA14930.15039017840324512No Hit
CCAATAATGTTCTCAAACAAAATGGCGAGACTGGGAAAAGGGTATATGTT14880.14988652743739367No Hit
ACTTACTGGTGGGATGGTCTTCAATCCTCTGACGATTTTGCTCTGATTGT14840.1494836066647125No Hit
GTTTATGTAAGACTTTTTCTTGCTCATATTGATTCCAAGTAGCTTACAGG14600.1470660820286255No Hit
CCTTTGGTACATTTGTTCATCTTCAAGTACTCCTCTTTGACTTGTATTCA14490.1459580499037523No Hit
GTCATATATGTGATCATGGCCAAAAACATCCGAGGATTCTGATTTTCGTT14410.14515220835839No Hit
ACACAATAGAAGTGTTCAGATCAAATGGCCTCACGGCCAATGAGTCTGGA14390.14495074797204938No Hit
CACAAATACAAACCCGAAGATCATTTGAAATAAAGAAACTGTGGGAGCAA14250.1435405252676653No Hit
CTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGG14080.14182811198377035No Hit
CCATTACATCCTTAAGGAAGTCTATGAGCCTTCCAGACTCATTGGCCGTG13990.14092154024523773No Hit
TTTGTATACTTTGTTGAGACACTGGCAAGGAGTATATGTGAGAAACTTGA13690.13789963445012898No Hit
CACATATACTCCTTGCCAGTGTCTCAACAAAGTATACAAACCCCCTTATT13550.13648941174574492No Hit
GTTTTGGGGTGTCTGGGATCAACGAGTCAGCGGACATGAGTATTGGAGTT13540.1363886815525746No Hit
ATTCTCAGGACACCGAACTTTCTTTCACCATCACTGGAGATAACACCAAA13420.13517991923453113No Hit
GGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTTTTCAA13390.13487772865502023No Hit
CCCTTTTCCCAGTCTCGCCATTTTGTTTGAGAACATTATTGGAGCAATAC13330.1342733474959985No Hit
GTTTATCATATTGTTTTTGATGACAGTAACTCCAATACTCATGTCCGCTG13250.13346750595063617No Hit
GTCTTCAATCCTCTGACGATTTTGCTCTGATTGTGAATGCACCCAATCAT13130.13225874363259266No Hit
GTGTTGGCCAATGCTGTTGCAGCAGGTTGGTTTCTATTTAGAGTCCAGTC13120.13215801343942238No Hit
GTATTCAAGATGGATCGATTTCTTTTGGGGATCCAGGAGTGTGTTGTTGC13100.1319565530530818No Hit
TATCTAATTAGAGCATTGACCCTGAACACAATGACCAAAGATGCTGAGAG13080.1317550926667412No Hit
CCTAATACAGTGCTTAACATATTGAACATGCCCATCATCATTCCAGGGCT13070.13165436247357093No Hit
ATTTAGAGTCCAGTCATAGGTCTGTCGGCCTTGTGTCAGCTTGTCTACTC13020.13115071150771948No Hit
TGATAACACAGAGAACAATAGGTAAAAAGAAGCAGAGATTGAACAAAAGG12970.130647060541868No Hit
GTTCTGTTTATGTAAGACTTTTTCTTGCTCATATTGATTCCAAGTAGCTT12940.13034486996235714No Hit
CCCATAACGATAGAAAAAACTTGTGAATTCAAATGTACCTGTTCTGTTTA12940.13034486996235714No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC12880.12974048880333539No Hit
ATCCCAGACACCCCAAAACTGGGGAGCTCCATGCTGAAATTGGCAACAAA12770.12863245667846218No Hit
ATGATAAACAATGATCTTGGTCCAGCAACAGCTCAAATGGCCCTTCAGTT12770.12863245667846218No Hit
GTAATGGAGTCAATGAACAAAGAAGAAATGGGGATCACAACTCATTTTCA12770.12863245667846218No Hit
GTGTTATCTCCAGTGATGGTGAAAGAAAGTTCGGTGTCCTGAGAATTGGT12770.12863245667846218No Hit
GTTGTAAGGAAGATGATGACCAATTCTCAGGACACCGAACTTTCTTTCAC12710.12802807551944043No Hit
AATTAGATAACTCCTTTTGTTCAATCTCTGCTTCTTTTTACCTATTGTTC12640.1273229641672484No Hit
ATGCAGTGATGATGCCAGCACATGGTCCAGCCAAAAACATGGAGTATGAT12500.12591274146286432No Hit
GTCTCTCACCCGTCTCTTTCTCTGAAAATGAGTTGTGATCCCCATTTCTT12310.12399886779262877No Hit
CAACAGGACACATCAGTACTCAGAAAAGGGAAGATGGACAACAAACACCG12280.1236966772131179No Hit
GTACTCAGAAAAGGGAAGATGGACAACAAACACCGAAACTGGAGCACCGC12230.12319302624726643No Hit
GTATTGCTCCAATAATGTTCTCAAACAAAATGGCGAGACTGGGAAAAGGG12130.12218572431556352No Hit
GTTCAGGGTCAATGCTCTAATTAGATAACTCCTTTTGTTCAATCTCTGCT11950.12037258083849828No Hit
GTTTCATACTCTTGCTCTCAAACATATACCCTTTTCCCAGTCTCGCCATT11910.11996966006581711No Hit
AAACAGAACAGGTACATTTGAATTCACAAGTTTTTTCTATCGTTATGGGT11810.1189623581341142No Hit
ACCCTGAACACAATGACCAAAGATGCTGAGAGAGGGAAGCTAAAACGGAG11670.1175521354297301No Hit
CCACCATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAG11650.11735067504338953No Hit
GTACGTGTACCTGTAATCTTTGATGAACAACTGAAGGGCCATTTGAGCTG11650.11735067504338953No Hit
CATTAGAAATCTCCACATTCCTGAAGTCTGCCTAAAATGGGAATTGATGG11610.11694775427070836No Hit
TCTTTGACTTGTATTCAAGATGGATCGATTTCTTTTGGGGATCCAGGAGT11560.11644410330485691No Hit
GTATACTTTGTTGAGACACTGGCAAGGAGTATATGTGAGAAACTTGAACA11500.11583972214583517No Hit
TCTCAGGACACCGAACTTTCTTTCACCATCACTGGAGATAACACCAAATG11430.11513461079364312No Hit
AGATAACACCAAATGGAACGAAAATCAGAATCCTCGGATGTTTTTGGCCA11380.11463095982779167No Hit
TTCCAGGGCTCAATGATGCAGTCCCCTCTATTAAGAGCGGTCGGATTTTT11370.11453022963462138No Hit
CTATGGCATCGGTACGTGTACCTGTAATCTTTGATGAACAACTGAAGGGC11370.11453022963462138No Hit
GTGTACCTGTAATCTTTGATGAACAACTGAAGGGCCATTTGAGCTGTTGC11350.11432876924828078No Hit
ATTCAAGATGGATCGATTTCTTTTGGGGATCCAGGAGTGTGTTGTTGCAA11310.11392584847559961No Hit
CCTTAAGGAAGTCTATGAGCCTTCCAGACTCATTGGCCGTGAGGCCATTT11260.11342219750974816No Hit
GTCTGGGATCAACGAGTCAGCGGACATGAGTATTGGAGTTACTGTCATCA11260.11342219750974816No Hit
ATATGTGATCATGGCCAAAAACATCCGAGGATTCTGATTTTCGTTCCATT11170.11251562577121554No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC11150.11231416538487497No Hit
ATACAGTGCTTAACATATTGAACATGCCCATCATCATTCCAGGGCTCAAT11090.1117097842258532No Hit
GAAAAGGGAAGATGGACAACAAACACCGAAACTGGAGCACCGCAACTCAA11070.11150832383951263No Hit
GATTTCTTGTCATATATGTGATCATGGCCAAAAACATCCGAGGATTCTGA11050.11130686345317203No Hit
ATCATATTGTTTTTGATGACAGTAACTCCAATACTCATGTCCGCTGACTC11020.11100467287366118No Hit
CCCTTATTTGCATCCCTGGGGTTGCAATTGCTCTCCGTTTTAGCTTCCCT10960.11040029171463941No Hit
GTATGAAACTTAGAACTCAAATACCTGCAGAAATGCTAGCAAGCATCGAT10870.1094937199761068No Hit
ATCATACTCCATGTTTTTGGCTGGACCATGTGCTGGCATCATCACTGCAT10840.10919152939659592No Hit
GAACAATAGGTAAAAAGAAGCAGAGATTGAACAAAAGGAGTTATCTAATT10800.10878860862391475No Hit
CCAATACTCATGTCCGCTGACTCGTTGATCCCAGACACCCCAAAACTGGG10760.1083856878512336No Hit
ATTGTTCTCTGTGTTATCATTTTCTTAGTCATATTGTCTCTCACCCGTCT10710.10788203688538213No Hit
TATTGATTCCAAGTAGCTTACAGGTTCGATAAAACCTGTCGACTCCGGCT10690.10768057649904156No Hit
AAATAAGGGGGTTTGTATACTTTGTTGAGACACTGGCAAGGAGTATATGT10630.10707619534001982No Hit
CTCCTGGATCCCCAAAAGAAATCGATCCATCTTGAATACAAGTCAAAGAG10600.10677400476050894No Hit
GCATCATTGAGCCCTGGAATGATGATGGGCATGTTCAATATGTTAAGCAC10550.10627035379465748No Hit
CCTATGACTGGACTCTAAATAGAAACCAACCTGCTGCAACAGCATTGGCC10550.10627035379465748No Hit
GACCAAAGATGCTGAGAGAGGGAAGCTAAAACGGAGAGCAATTGCAACCC10440.10516232166978426No Hit
CTCCAGTGATGGTGAAAGAAAGTTCGGTGTCCTGAGAATTGGTCATCATC10420.10496086128344369No Hit
GAACAGGTACATTTGAATTCACAAGTTTTTTCTATCGTTATGGGTTTGTT10420.10496086128344369No Hit
CCTGTAATCTTTGATGAACAACTGAAGGGCCATTTGAGCTGTTGCTGGAC10370.10445721031759224No Hit
TCTTTGGTCATTGTGTTCAGGGTCAATGCTCTAATTAGATAACTCCTTTT10230.10304698761320814No Hit
ATCACATATATGACAAGAAATCAGCCCGAATGGTTCAGAAATGTTCTAAG10200.10274479703369728No Hit
GCATAAACGCCCCTGGTAATCCTCATCCATCAATTCCCATTTTAGGCAGA10160.10234187626101611No Hit
GATTACAGGTACACGTACCGATGCCATAGAGGTGACACACAAATACAAAC10150.10224114606784583No Hit
TTCTTTCACCATCACTGGAGATAACACCAAATGGAACGAAAATCAGAATC10140.10214041587467551No Hit
GTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTC10130.10203968568150523No Hit
GCATTGTTCATTGATTCAATTTCTTTATGGCTGACAAATGGGTTCAGTGG10100.10173749510199435No Hit
CTCCATTACATCCTTAAGGAAGTCTATGAGCCTTCCAGACTCATTGGCCG10040.10113311394297261No Hit
CGCCTAATACAGTGCTTAACATATTGAACATGCCCATCATCATTCCAGGG10010.10083092336346172No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)