FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S16_R1_E07-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S16_R1_E07-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819019
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG372914.553130025066573No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA371814.539699323214724No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA331644.049234511043089No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA186182.2732073370703243No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA168512.057461426413795No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC148881.817784447003061No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT140421.7144901400333816No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC138911.6960534493094785No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG116781.4258521475081773No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGC115811.4140087104206374No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG110501.3491750496630726No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT108571.3256102727775547No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG105391.2867833346967532No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT95821.1699362285856616No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC94111.1490575920705137No Hit
GCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGA90351.1031490111951006No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG89321.0905729903701868No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT80740.9858135159257599No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC77040.9406375187877205No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC69200.8449132437709015No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG68440.8356338497641691No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGT66820.8158540888550815No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA64810.7913125336530654No Hit
CAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCG63560.7760503724577819No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC63240.7721432591917892No Hit
CATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATG61780.754317054915698No Hit
GTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGATATTT60540.7391769910099766No Hit
CATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGT60270.7358803641917954No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA58980.7201298138382626No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA52980.6468714401009013No Hit
AATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGA49970.6101201559426582No Hit
GAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAA48670.5942475082995633No Hit
CTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG47840.5841134332658949No Hit
GTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGA45650.557374126851758No Hit
CACAAAAACCACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAG45560.5562752512456975No Hit
ACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGA42490.518791383350081No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCG42270.5161052429797112No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA41700.5091456974746618No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACC41560.50743633542079No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC41260.503773416733922No Hit
GCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA40750.4975464549662462No Hit
ATCCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCATTTCAT39410.4811854181649021No Hit
ACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACA39320.48008654255884176No Hit
GCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACT39210.47874347237365683No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAA38730.47288280247466785No Hit
TCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCA38070.46482438136355814No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT37610.4592079060436937No Hit
CCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGG37370.45627757109419925No Hit
ATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATC36460.4451667177440328No Hit
GAAATATCCAATTACAGCAGACAAGAGGATAACGGAAATATGGGCCAGCA36280.442968966531912No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACC35950.4389397559763571No Hit
GCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAA35660.43539893457905127No Hit
CACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAA33860.41342142245784286No Hit
CAAAAACCACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGA32280.3941300507070044No Hit
GAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATCC31550.3852169485689587No Hit
TAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGG30920.3775248193265358No Hit
ATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCA30470.37203044129623364No Hit
ATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGCCC29350.35835554486525956No Hit
GAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAAT29180.35627989094270096No Hit
ACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAG28860.3523727776767084No Hit
GGATGAAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGG28280.3452911348820968No Hit
CACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGT27730.338575783956172No Hit
GGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATC27520.3360117408753643No Hit
CATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGT26900.32844170892250363No Hit
TTACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT25900.3162319799662767No Hit
GGATATTTCATTGCCATCATCCATTTCATCCTAAGTGCTGGGTTCTTCTC25820.31525520164977855No Hit
AGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCC25620.3128132558585332No Hit
AAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCA25240.30817355885516695No Hit
TCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTTCCGT25060.30597580764304616No Hit
AAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGGATAAC23890.29169042476426066No Hit
ACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTT23880.2915683274746984No Hit
GGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCG23330.2848529765487736No Hit
CTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTC22650.2765503608585393No Hit
GGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGA22420.27374212319860713No Hit
GGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGATATTTCATTG22340.27276534488210896No Hit
AAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTT21960.26812564787874277No Hit
CAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCA21760.26568370208749736No Hit
CCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTT21490.2623870752693161No Hit
GGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAG21420.26153239424238023No Hit
CCGTTATCCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCAT21110.25774737826594984No Hit
CCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCATTTCATCC20940.2556717243433913No Hit
GAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGT20880.2549391406060177No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTG20500.25029944360265144No Hit
GGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCAC20400.24907847070702877No Hit
TTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGC20210.24675862220534567No Hit
CCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCATAATCAAG20050.24480506557234935No Hit
GTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTC19620.2395548821211718No Hit
GAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAA18980.23174065558918658No Hit
GCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTT18790.22942080708750348No Hit
GTAATTGGATATTTCATTGCCATCATCCATTTCATCCTAAGTGCTGGGTT18770.22917661250837895No Hit
TACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT18480.22563579111107313No Hit
GTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGCCCAATTAGCA18390.2245369155050127No Hit
TCCAATTACAGCAGACAAGAGGATAACGGAAATATGGGCCAGCACTAAGC18320.2236822344780768No Hit
TAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTC18230.2225833588720164No Hit
AGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTG17900.21855414831646153No Hit
CTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGCCCAAT17670.21574591065652934No Hit
GCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTG17650.21550171607740481No Hit
CAAGAGGATAACGGAAATATGGGCCAGCACTAAGCATCAATGAACTGAGC17390.2123271865487858No Hit
GCACTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACAGCAGA17150.20939685159929136No Hit
CAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATA17070.20842007328279322No Hit
GAAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGGATAA17000.20756539225585732No Hit
TCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATAT16630.20304779254205338No Hit
CATCCATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTCTTCCTGATGTGT16520.2017047223568684No Hit
AAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC16380.19999536030299664No Hit
GTGCTGGGTTCTTCTCCTGTCTTCCTGATGTGTACTTCTTGATTATGGCC16260.19853019282824938No Hit
CAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCA16180.19755341451175124No Hit
ATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGAT16010.1954777605891927No Hit
CCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAG15930.19450098227269452No Hit
CAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCT15910.19425678769357No Hit
GTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTC15850.19352420395619638No Hit
GCTGTAATTGGATATTTCATTGCCATCATCCATTTCATCCTAAGTGCTGG15700.19169274461276234No Hit
CTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAA15660.19120435545451328No Hit
GGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGGATAACGGAA14680.1792388210774109No Hit
ATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTCTTCCTGATGTGTACTTC14320.1748433186531692No Hit
GACAAGAGGATAACGGAAATATGGGCCAGCACTAAGCATCAATGAACTGA14170.1730118593097352No Hit
GCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGG13820.16873845417505579No Hit
GGATAACGGAAATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCT13800.16849425959593123No Hit
GTTCTTCTCCTGTCTTCCTGATGTGTACTTCTTGATTATGGCCATATGGT13400.16361036801344045No Hit
TTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTAT13220.16141261680131963No Hit
GACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAA13160.16068003306394602No Hit
TCATTACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCT12980.15848228185182517No Hit
GAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACC12960.15823808727270064No Hit
TCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGC12880.15726130895620247No Hit
TATTTCCGTTATCCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCA12700.15506355774408165No Hit
CGGAAATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAA12420.1516448336363381No Hit
TTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAA12410.15152273634677582No Hit
TTTCATTGCCATCATCCATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTC12250.14956917971377953No Hit
CTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAAC11950.14590626102691145No Hit
AATTACAGCAGACAAGAGGATAACGGAAATATGGGCCAGCACTAAGCATC11770.14370850981479064No Hit
ATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAG11730.14322012065654155No Hit
CAATTACAGCAGACAAGAGGATAACGGAAATATGGGCCAGCACTAAGCAT11610.14175495318179432No Hit
CTGTAATTGGATATTTCATTGCCATCATCCATTTCATCCTAAGTGCTGGG11490.1402897857070471No Hit
GCCATCATCCATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTCTTCCTGA11450.13980139654879803No Hit
GATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGGATAACGGAAA11320.13821413178448852No Hit
TTTCATTACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCT11310.13809203449492624No Hit
TGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTC11200.1367489643097413No Hit
GACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAA11110.13565008870368087No Hit
CTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCC10980.13406282393937138No Hit
GTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCC10830.13223136459593735No Hit
TGTCTGCTGTAATTGGATATTTCATTGCCATCATCCATTTCATCCTAAGT10670.13027780796294103No Hit
CTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCATTTCATCCT10590.12930102964644288No Hit
CTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGC10550.1288126404881938No Hit
GTTATCCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCATTT10520.128446348619507No Hit
CAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGA10230.12490552722220119No Hit
GTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATA10180.12429504077438985No Hit
ATCCAATTACAGCAGACAAGAGGATAACGGAAATATGGGCCAGCACTAAG10140.12380665161614078No Hit
AAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATC9950.12148680311445766No Hit
GAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATCCAA9840.1201437329292727No Hit
TGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGG9620.11745759255890278No Hit
ATTACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCC9620.11745759255890278No Hit
GCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTA9410.11489354947809513No Hit
TTCCTGATGTGTACTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTT9340.11403886845115926No Hit
CTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCATTTCATCCTAA9210.11245160368684975No Hit
GCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGCGGGTGCGAGACTG9140.11159692265991389No Hit
GCGAGATACTCACAAAAACCACCGTGGACCATATGGCCATAATCAAGAAG9130.11147482537035161No Hit
GCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCA9080.11086433892254026No Hit
AACCACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGAC8860.10817819855217035No Hit
GTGGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAA8860.10817819855217035No Hit
CGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCATAATCAAGAA8840.10793400397304581No Hit
ACTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACAGCAGACA8520.1040268907070532No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)