FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S16_R1_E06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S16_R1_E06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937083
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGT20550.21929754354736986No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT19320.20617170517446162No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG17870.19069815587306566No Hit
GGATGTAATCATGGAAGTTGTTTTCCCTAACGAAGTGGGAGCCAGGATAC17800.18995115694127415No Hit
GCCTAAGGATGTCTACCATCCTAATTCCACCAATCTGTGTGCTGTGGCAC15960.17031575644846828No Hit
AACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGAG15450.16487333565970144No Hit
ATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAA14890.15889734420536922No Hit
ATTACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGACCAGGATTTAT14550.15526906367952464No Hit
GTATTTGTTATTGGTCCATTCCTATTCCACCATGTCACAGCCAGAGGTGA14330.152921352751037No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA14230.15185421141990624No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA14090.15036021355632317No Hit
CTTCATATCCCTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTA13660.14577150583246096No Hit
GATGTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCT13330.14224993943972947No Hit
GCATATATCCACGGCTTGCTCTTCTGTTGGGTTCTGCCTAAGGATGTCTA11640.12421525094361972No Hit
ATATAGCGGTATTCCCTCTCCTTCATGACTTGGGTCCTCAATGCTTAATT11410.12176082588201899No Hit
GTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATT11080.1182392594892875No Hit
GATATATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTT10980.11717211815815674No Hit
CCACTACCCTCTCCGTGCTGGAGTACTCATCTACACCCATTTTGCTGATT10860.11589154856079985No Hit
GGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCCGGGACCAACGAGGA10860.11589154856079985No Hit
GTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTCAAT10820.11546469202834754No Hit
TAATAAAACTTCTTCCCTTCGCAGCCGCTCCACCAAAGCAAAGTAGAATG10530.11236998216806834No Hit
CAATTATTGCTTCGGCAATCGACTGTTCGTCTCTCCCACTCACTATCAGC10440.11140955497005067No Hit
GAATGAGGAATCCCCTCAGAACAGCGGACTCCACTCCAGCTGTGCCTTCA10100.10778127444420613No Hit
GCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTG10090.10767456031109304No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA10070.1074611320448669No Hit
GTAGTACATTTCCTCGTTGGTCCCGGATTCTCAAAAAACGGTCAATGCTC10060.10735441791175382No Hit
CTCCAACATGTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTT10000.10671413311307537No Hit
CTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTC9780.1043664221845877No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGA9650.10297913845411773No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA9480.10116499819119544No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT9450.10084485579185622No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)