FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S16_R1_D04-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S16_R1_D04-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560633
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTATATAGTACATATGATCCCCAGTAGGATACCATTGTCTTAATGCA16570.2955587701758548No Hit
ATATAGTACAGGCACCATAGTCCTCTTCTAAAATCCAGCAAATTCCAAAT15020.26791145009301987No Hit
ATATAGTACATATGATCCCCAGTAGGATACCATTGTCTTAATGCATCAAC13730.24490174499182177No Hit
TATATATAGAGAGAGATGGATCGATCAAAACACATCAAGTTATCCACCTG10270.18318579177465472No Hit
GTTGTTATTAGGGTGACTTCCCGCTAAGATTTAGTTCTTCTTTAAATGGT10060.17944002582794805No Hit
GTCTTAATGCATCAACATTCCTGCAGTCAAATGTTTGAATCCTTGTATCA9790.1746240410393252No Hit
GATATATATAGAGAGAGATGGATCGATCAAAACACATCAAGTTATCCACC9280.16552718088303758No Hit
TTATTAGGGTGACTTCCCGCTAAGATTTAGTTCTTCTTTAAATGGTTTCT8740.15589521130579184No Hit
CTATTAAGTCTGTTTTCACATTCGCCGCCTTCTCCTTTCCCAAGAGAAGG7900.14091214751896516No Hit
TCATAAGACAGAGCAACCTACTGTCCTCTATCCACAATTCCAAATTTTAA7790.13895007964211883No Hit
GTACATATGATCCCCAGTAGGATACCATTGTCTTAATGCATCAACATTCC7750.13823660041417468No Hit
TCCTAATACTGTAACACTTGAAGAGATGGATCACCATTAGTAATCTTTAC7580.13520431369541214No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT7470.1332422458185658No Hit
GTCTTATGAGCCCCATTTTATTAGTGAGGAAACCCAGGTACAGAGCATAT7430.13252876659062168No Hit
GTGTTGAGAAGTAACAATAAAGCAGGGAAAGAGGAATGAATCTTTGGGGT7220.128783000643915No Hit
GTACTATATAGTACAGGCACCATAGTCCTCTTCTAAAATCCAGCAAATTC7020.12521560450419436No Hit
GTCCAATGTGGGTCTTGATCCCAGGATCCTGAGATCATGACCTGAGTTGA6830.12182657817145977No Hit
ATCTTAGGGGGAGAAAGGCTGCTGCCCGTGCAGGTCTGGGTCCTGTCCAT6470.11540526511996262No Hit
TATATAGAGAGAGATGGATCGATCAAAACACATCAAGTTATCCACCTGAA6090.10862721245449339No Hit
ATACCATACGGAGCACAGGATGAGATTTGAACTTGGGCAGTCTGCTCCAG6060.10809210303353531No Hit
TAGATATGCTCTGTACCTGGGTTTCCTCACTAATAAAATGGGGCTCATAA6000.10702188419161912No Hit
GTATGAAAAGCTGCACCAGCTGCTGGACGAGGACCTGCGGCAGACGGTGG5960.106308404963675No Hit
GTTGTGTATCTTCCCTTCTATTAAGTCTGTTTTCACATTCGCCGCCTTCT5940.10595166534970292No Hit
CTTTATAGGTTTCTCTAAATGTTGAACTTCAGGTCAAATTGGGTGAGAAC5910.10541655592874483No Hit
ATTAAATACCATACGGAGCACAGGATGAGATTTGAACTTGGGCAGTCTGC5770.10291937863094038No Hit
GTCTTTAATTGGGAAATGAAATATTGTTGTTAACTGCCCAACAACAGGTC5680.10131405036806611No Hit
GGGTAGGAGAACTAAGTGATTGTTGCCAAGATTTGTCATTACATTAGTGA5620.1002438315261499No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)