FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S16_R1_C12-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S16_R1_C12-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947799
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAACACAATGTAACACAATGCTTGACTTCAGGGGAAATACTCAACCA20160.2127033263381793No Hit
ATGTAACACAATGCTTGACTTCAGGGGAAATACTCAACCAAAAAATCTAG15570.1642753368593974No Hit
GTGTTACATTGTGTTACATTGGGCCTTACCTCATCTGGTCTTATCAGCAG15300.16142663159593965No Hit
ATGTAACACAATGTAACACAATGTAACACAATGCTTGACTTCAGGGGAAA13660.14412338481049253No Hit
GTGTTACATTGGGCCTTACCTCATCTGGTCTTATCAGCAGGGCTGTGAAA12350.13030188890260488No Hit
TCCCCGAAGTAACTCATTCACCCGTTAAATAAATAGTGATCCAACATCAA11870.12523752398979107No Hit
CTCCCTAAAGGGACAAGATGTTATAAAAAGAAAGAAATACATTGACAGCG11200.11816851463232184No Hit
CTTTAGCGAGTCTCATCGGCTGCAAATCCATAAAACACCCTGCACTTCCA10870.11468676375476233No Hit
TCTTATATGTTCTTGATTTTAAGACAAAGGCTGCATGATTTGAGCTAATG10550.11131052047955316No Hit
GATATATATAGAGAGAGATGGATCGATCAAAACACATCAAGTTATCCACC10240.10803978480669425No Hit
CCACAGGGCCAGTCCCTGAGTCTGTCACATACACAGCAATACAAGCTTCT9510.10033772983512328No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)