FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S12_R1_G12-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S12_R1_G12-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19939
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAAGAAGGGCATCACCTTCTTGGCCCAGCTTTTGTTGCGCCTGCGCAT700.3510707658357992No Hit
GCCATGGGCGTGTCTGTGATCGAAGCGCTGATAGCGCTGGCCACGCTGGG680.34104017252620494No Hit
ATATAAGGGCGTCTATAAGAGCCTTCCTAAATCCTTATATATATTGAAAA660.33100957921661067No Hit
CCCTATGTCCTCCCTGCCTGTTGCCGCAGCAACATCGGTATTCTGGCAAG610.305933095942625No Hit
GTATACGCGGGCCTGCTCATCCATCCAAAGCACTACGCTTTGCAGGGCAA600.30091779928782786No Hit
GTCATATCCCAGCGACAGGTCTTTTTTACCTTTCGCCAAGATTTTCTGCA580.2908872059782336No Hit
GCTTTTGTTGCGCCTGCGCATGGCGTGGGCTTTGCGTGCCGTTGGGGCTG560.2808566126686393No Hit
GGATAATGAAGAGCCGATGCAGGCCCAAGACCTCACCGAAGGGCTGCAAG540.2708260193590451No Hit
GATACGCCCCATGCCATGCACGACGACCCGTACCGAGTATCCGCTGGGTG540.2708260193590451No Hit
GACTAGAGCAGTTCGAAGGGGGCATATTTGTAATTCATTAGCGTCATTCA510.2557801293946537No Hit
CCCTTATATAGTCTTAAAAACTGGGGTTTTTGCCGGGAATACCGGGAAAG510.2557801293946537No Hit
TCATTATCCTGTCCTTGGGCGCCTGCTGCCGACGCCAGCTGTTCTAACAC500.25076483273985656No Hit
GTATTATAAGACGCCCGGACAACTTCGGCTACTGTCGCTGCAAATAATTT490.24574953608505942No Hit
GTCTTTACACCGAATGCATGTTATTTGGCGATTACGACCATAATCAAAGC480.2407342394302623No Hit
GCTCATACCTTTCCCCTTACAAAGTCAGTTGGACGATGGTCAGACCTGCA470.23571894277546515No Hit
GTGCTACGGCTGGCACATGGCTGGGGGCTGCGACTGTACGCTCCAATCTG440.2206730528110738No Hit
GGTTACGACGCGCGGCGCGCCATCGATGTGGCACCCAGATTGGAGCGTAC430.21565775615627666No Hit
GCCATGTGCCAGCCGTAGCACCACCGCCCCGGGCCGGCGCGCTGGAAACG420.21064245950147953No Hit
CCTTGGTGCACAACGATTTGCGCCTTGCGCAGGCGGAGGCACGCAAGCGC410.2056271628466824No Hit
ACCTCATACGGTTGACCTTCCCAAACGCCGGGTTTTTCTTCGAAGTCGCA410.2056271628466824No Hit
CCCCCAGCGTGGCCAGCGCTATCAGCGCTTCGATCACAGACACGCCCATG410.2056271628466824No Hit
TTATAATACCGATCATCTAGTTATAGCGACGTACACTTTCATGCCATCTG390.19559656953708812No Hit
ACCCTAGACTTTGGGAGGGTCCGAAGGCTGATCAGGGGCCTCCCGGCTGG380.190581272882291No Hit
GTCACTGCCAACGTTTCGGGGCTGGCAGGAGGTCCATCGATTGCGCTCGG370.18556597622749388No Hit
GTCCGGTTTGGCGCTTGCGTGCCTCCGCCTGCGCAAGGCGCAAATCGTTG370.18556597622749388No Hit
CCGTAGCACCACCGCCCCGGGCCGGCGCGCTGGAAACGGACGGTCAGTCC370.18556597622749388No Hit
TAGCACCACCGCCCCGGGCCGGCGCGCTGGAAACGGACGGTCAGTCCGGT370.18556597622749388No Hit
GTCTACAGCAGCTGGGTCTGTGATCGGGTCCGCCGATACAGGGTCTTCGC370.18556597622749388No Hit
GCCGTACCCCATTCCCTATGTCCTCCCTGCCTGTTGCCGCAGCAACATCG370.18556597622749388No Hit
AGATTGGAGCGTACAGTCGCAGCCCCCAGCCATGTGCCAGCCGTAGCACC370.18556597622749388No Hit
GGCTACAGCCAGCGCAACGGCAGTCTTCAGCACGGGGGCTCCGGGAAAGA360.18055067957269674No Hit
CGCTGGGGGTGTTGTTGGCGCTGTCACTGCCAACGTTTCGGGGCTGGCAG360.18055067957269674No Hit
GCAGGGAGGGATCCCGGCAAGCGGAGGGCTGGCACTCGGGACGCACGAGC360.18055067957269674No Hit
ATCCAATACTCTTGCGCCCGGCACGCGACCCTCACGCACTAGTGCGATGT350.1755353829178996No Hit
TAATAAACGTTCCCGGTCAAAACGCCGTCCAAGCCTTTGTCTTCTGGCGC350.1755353829178996No Hit
GGCAGGAGGTCCATCGATTGCGCTCGGCGTCCACCTTGGTGCACAACGAT350.1755353829178996No Hit
GGTAAAGGGCTGCCCTTTATCATCTTTTGGCATCTTTGTTTATTCGGCAG350.1755353829178996No Hit
GTGTAAAGACCTTCCCGGCAAGATGGATCGCCGCTTGCCGGGAAGGTATC350.1755353829178996No Hit
GCCATGCACGACGACCCGTACCGAGTATCCGCTGGGTGACTGCAGCGTGA340.17052008626310247No Hit
GTCGCAGCCCCCAGCCATGTGCCAGCCGTAGCACCACCGCCCCGGGCCGG340.17052008626310247No Hit
GGATATGACTGCGACTTCGAAGAAAAACCCGGCGTTTGGGAAGGTCAACC340.17052008626310247No Hit
CAGGTGTACCGCCTGCGCATCACGTCCCTCGGCAAGCTGATCCTGACCAA340.17052008626310247No Hit
CCGTTGGGGCTGGATGGTCGCCAAGATCCCGGCCGATGGGAACGCAACCC330.16550478960830534No Hit
GGTGTACCGCCTGCGCATCACGTCCCTCGGCAAGCTGATCCTGACCAAGT330.16550478960830534No Hit
GGGTCAAGAAGGGCATCACCTTCTTGGCCCAGCTTTTGTTGCGCCTGCGC330.16550478960830534No Hit
GTATCGACTTCGTCACCGTCGAAGCCCGAAAGCATCTCGTGATCATCGAT330.16550478960830534No Hit
GGGTACGGCGGTGCGCCACGCCCAGCCATGGGCGTGTCTGTGATCGAAGC330.16550478960830534No Hit
GTTCTAACACGGCCTGCGCCTGCGAGATAAGCGCGGCAAGGTCGGGAGCG320.1604894929535082No Hit
AAACCGGACTGACCGTCCGTTTCCAGCGCGCCGGCCCGGGGCGGTGGTGC320.1604894929535082No Hit
GTGCCAGCCGTAGCACCACCGCCCCGGGCCGGCGCGCTGGAAACGGACGG320.1604894929535082No Hit
GTTATATCCCGAACAACGTCGACTCGACCAAAGAGACTACCGGAATCCCG320.1604894929535082No Hit
GCTATGGACGCACGTGGCGTTATTCGCTACGGGGTGAAAAAGCTGGGTAT310.15547419629871106No Hit
GTCCATGGTCTTAACTTTAACGCCCATGGTGTTCTCCTTATCGGATGGTG310.15547419629871106No Hit
CGTTTATTATGAGAAGCCGTGGATGCGCGGCGACCTCAAAGTGTTTTCCC310.15547419629871106No Hit
CTATTGCCTCTTTCATGAACGTTCCTTTGATCGATGATCACGAGATGCTT300.15045889964391393No Hit
GCCCCCAGCCATGTGCCAGCCGTAGCACCACCGCCCCGGGCCGGCGCGCT300.15045889964391393No Hit
CTTCAATCCAGCTGGGCGTGGCTTGAAATGAATTTTTCAGGGGCGACAAC300.15045889964391393No Hit
AAAGCAGGGAGGGATCCCGGCAAGCGGAGGGCTGGCACTCGGGACGCACG300.15045889964391393No Hit
GTGTACCGCCTGCGCATCACGTCCCTCGGCAAGCTGATCCTGACCAAGTA300.15045889964391393No Hit
CCTTTGATCGATGATCACGAGATGCTTTCGGGCTTCGACGGTGACGAAGT300.15045889964391393No Hit
CTTCAGCACGGGGGCTCCGGGAAAGAGCGCGGGCTGGCTGAGGCTGGCTG300.15045889964391393No Hit
GTATTGGTCGATGTCTGGAGACGACGGAACAGCCGCATCGGCTGCGCGAG290.1454436029891168No Hit
GTAGGGGCCAGGGCGGCGAGGGTCTGGCCAGTCGCCGGAACTTCGCCGGT290.1454436029891168No Hit
GGCTTGGACGGCGTTTTGACCGGGAACGTTTATTATGAGAAGCCGTGGAT290.1454436029891168No Hit
GGCGTGGGCTTTGCGTGCCGTTGGGGCTGGATGGTCGCCAAGATCCCGGC290.1454436029891168No Hit
GTACAGTCGCAGCCCCCAGCCATGTGCCAGCCGTAGCACCACCGCCCCGG290.1454436029891168No Hit
AAACAGGGGATGGCGAGCCCATGACCGCCTTGCCAGAATACCGATGTTGC290.1454436029891168No Hit
ACGTTGGGCTGCAGGTCGATATCCGGCCAATCGTCGCTGGTTACGACGCG290.1454436029891168No Hit
ATCCCGAACAACGTCGACTCGACCAAAGAGACTACCGGAATCCCGTTCGG280.14042830633431966No Hit
GGGCTGGATGGTCGCCAAGATCCCGGCCGATGGGAACGCAACCCGCTTCA280.14042830633431966No Hit
CTCTTCCGCTCCCCGAAACAGGGGATGGCGAGCCCATGACCGCCTTGCCA280.14042830633431966No Hit
CTCTAAAGTTGTTGGGGCCTTTGATCACAAAGCTATGGACGCACGTGGCG280.14042830633431966No Hit
CTCCAATCTGGGTGCCACATCGATGGCGCGCCGCGCGTCGTAACCAGCGA280.14042830633431966No Hit
GTATTGGATGGTCGTTGTTTTGATCATCTCAGTTTTGATTTCACACCATC280.14042830633431966No Hit
ATTATGTTCTGAGTGCAACTAAGCAGACAACTAGCGGCCACATATTGACT270.13541300967952255No Hit
GCTGGGGGCTGCGACTGTACGCTCCAATCTGGGTGCCACATCGATGGCGC270.13541300967952255No Hit
GTCATGGGCTCGCCATCCCCTGTTTCGGGGAGCGGAAGAGCGCTCCGAAG270.13541300967952255No Hit
GTCCAGACTCGCGCAGCCGATGCGGCTGTTCCGTCGTCTCCAGACATCGA270.13541300967952255No Hit
AGTATCCGCTGGGTGACTGCAGCGTGACGTTGCCAGCGGTGATGGTTCCG270.13541300967952255No Hit
GGCGGAGGCACGCAAGCGCCAAACCGGACTGACCGTCCGTTTCCAGCGCG260.13039771302472541No Hit
CCCGTACCGAGTATCCGCTGGGTGACTGCAGCGTGACGTTGCCAGCGGTG260.13039771302472541No Hit
GGCTTTGCGTGCCGTTGGGGCTGGATGGTCGCCAAGATCCCGGCCGATGG260.13039771302472541No Hit
CCTTCTTGGCCCAGCTTTTGTTGCGCCTGCGCATGGCGTGGGCTTTGCGT260.13039771302472541No Hit
GTCTATGATAGACCCGGTTTCTTCAGCTTTAAATGGGCTGCACTTGATAT260.13039771302472541No Hit
GGCAGTGACAGCGCCAACAACACCCCCAGCGTGGCCAGCGCTATCAGCGC250.12538241636992828No Hit
GGTGGACGCCGAGCGCAATCGATGGACCTCCTGCCAGCCCCGAAACGTTG250.12538241636992828No Hit
GGTCGATATCCGGCCAATCGTCGCTGGTTACGACGCGCGGCGCGCCATCG250.12538241636992828No Hit
GGTAAACGTGTACCGCCCGGAATCGGCAGTTAGCGACCCTCGCGCTATTG250.12538241636992828No Hit
GCAAATAACCATGCCATTTCAAACTGTTGTTAATCGCAACTTCACCACTG250.12538241636992828No Hit
AGCGAAAGCAGGGTCAAGAAGGGCATCACCTTCTTGGCCCAGCTTTTGTT250.12538241636992828No Hit
ATATCCGGCCAATCGTCGCTGGTTACGACGCGCGGCGCGCCATCGATGTG250.12538241636992828No Hit
TCTTGGCCCAGCTTTTGTTGCGCCTGCGCATGGCGTGGGCTTTGCGTGCC250.12538241636992828No Hit
GACCCGTACCGAGTATCCGCTGGGTGACTGCAGCGTGACGTTGCCAGCGG250.12538241636992828No Hit
ATCATAGACCCTTATGAATGACGCTAATGAATTACAAATATGCCCCCTTC250.12538241636992828No Hit
GTTATTTGCTCCCCTGCGCGATGTATTGGTCGATGTCTGGAGACGACGGA240.12036711971513114No Hit
GCCCCATGCCATGCACGACGACCCGTACCGAGTATCCGCTGGGTGACTGC240.12036711971513114No Hit
GCCTGGTGTTCTGGCTCTTTGGCCTCTTCGGACATGAACTGTTCAAGCGC240.12036711971513114No Hit
CCGATAAGGTTATCCCAAGAAAATCCCGAACCCTGCGGCCCGCCTATCTC240.12036711971513114No Hit
CCCTAGCACGGCGAGCGATCCGACACAAGATGAAGGCCCGACAACGGCTC240.12036711971513114No Hit
GCTACAGCCAGCGCAACGGCAGTCTTCAGCACGGGGGCTCCGGGAAAGAG240.12036711971513114No Hit
GACATGAACTGTTCAAGCGCTTGGGTGAGTTGCGGAATCAGCGTTTGAAG240.12036711971513114No Hit
CCCCAGCCATGTGCCAGCCGTAGCACCACCGCCCCGGGCCGGCGCGCTGG240.12036711971513114No Hit
CCACCGCCCCGGGCCGGCGCGCTGGAAACGGACGGTCAGTCCGGTTTGGC240.12036711971513114No Hit
GTCTTAACTTTAACGCCCATGGTGTTCTCCTTATCGGATGGTGTGAAATC230.11535182306033401No Hit
GTACCGAGTATCCGCTGGGTGACTGCAGCGTGACGTTGCCAGCGGTGATG230.11535182306033401No Hit
TTGCAGGGCAACTCGGTTGACGGCCCACTGGGCGCCTCGCTGGCAGTGCC230.11535182306033401No Hit
CCAAGATCCCGGCCGATGGGAACGCAACCCGCTTCAATCCAGCTGGGCGT230.11535182306033401No Hit
ATGTATTGGTCGATGTCTGGAGACGACGGAACAGCCGCATCGGCTGCGCG230.11535182306033401No Hit
TGCCAGCGGTGATGGTTCCGCGCGCCGGGTTGATCCCGAAAATGCCCTCG230.11535182306033401No Hit
CCTCCTGCCAGCCCCGAAACGTTGGCAGTGACAGCGCCAACAACACCCCC220.1103365264055369No Hit
GCGCAAATCGTTGTGCACCAAGGTGGACGCCGAGCGCAATCGATGGACCT220.1103365264055369No Hit
CCATCGATGTGGCACCCAGATTGGAGCGTACAGTCGCAGCCCCCAGCCAT220.1103365264055369No Hit
CATATTGACTGGCGCAAATTATTTGCAGCGACAGTAGCCGAAGTTGTCCG220.1103365264055369No Hit
GGATATAACCGACAAAGGATGCAGGGGTAACGGCCTGCACTGCCAGGGTG220.1103365264055369No Hit
CGGCAACGTTGGGCTGCAGGTCGATATCCGGCCAATCGTCGCTGGTTACG220.1103365264055369No Hit
ATGTGGCACCCAGATTGGAGCGTACAGTCGCAGCCCCCAGCCATGTGCCA220.1103365264055369No Hit
GGCCAGACCGGCCACGGCTTGATCGGTTACTTGCTTCATCTCCAGCTGCC220.1103365264055369No Hit
CCCAAGGACAGGATAATGAAGAGCCGATGCAGGCCCAAGACCTCACCGAA220.1103365264055369No Hit
TGCCAAGGCTATCCGCATCAGCGCTCTGAGTGCTGCGCAAAAGGCCAACG220.1103365264055369No Hit
GTCTGTGATCGAAGCGCTGATAGCGCTGGCCACGCTGGGGGTGTTGTTGG220.1103365264055369No Hit
GTGTAGCCGTAAACGCGACCAATCAGGTTGCCGGAGAAACGGGCGTTGGC220.1103365264055369No Hit
CTTCACCACTGGCTTTCCCGGCGAAATTGTTCGTGACGGCCCAACTCGTG220.1103365264055369No Hit
AGTCGATACCGCGCCAGAAGACAAAGGCTTGGACGGCGTTTTGACCGGGA220.1103365264055369No Hit
GATTTACAGTGCCCTGAGTGACGCCCGAATGGAAGCGAACGGGGTTGAGG220.1103365264055369No Hit
GGTCAGACCTGCAACCGGCGCACCAACAGCGGACGCGGACAGGCTGATCG220.1103365264055369No Hit
GTCCAAGCCTTTGTCTTCTGGCGCGGTATCGACTTCGTCACCGTCGAAGC220.1103365264055369No Hit
AGTCTGGACGGTTTTGCGGGCTGCTGCCTGGGCCTTTTCGGTGGCGTTCA220.1103365264055369No Hit
GGGTTGATCCCGAAAATGCCCTCGGCAACGTTGGGCTGCAGGTCGATATC220.1103365264055369No Hit
GGGGGTGTTGTTGGCGCTGTCACTGCCAACGTTTCGGGGCTGGCAGGAGG220.1103365264055369No Hit
ACCATGGACAAGGCGACGCGTGATAGCGCCGTTGAATCTCTTCAAACGCT220.1103365264055369No Hit
GTCTAGGGTCGTTCAACTTGAAGATCATGCCGTCCTTTTCAACGTGAGTG220.1103365264055369No Hit
GCCCATGACCGCCTTGCCAGAATACCGATGTTGCTGCGGCAACAGGCAGG210.10532122975073976No Hit
GTGCACAACGATTTGCGCCTTGCGCAGGCGGAGGCACGCAAGCGCCAAAC210.10532122975073976No Hit
CAACACCCCCAGCGTGGCCAGCGCTATCAGCGCTTCGATCACAGACACGC210.10532122975073976No Hit
ATTCTGCGATTAGCGGTTTCTACGCTGATCTTGCTCTTAAGGATGGCATC210.10532122975073976No Hit
GTTGTTGGCGCTGTCACTGCCAACGTTTCGGGGCTGGCAGGAGGTCCATC210.10532122975073976No Hit
GTATGAGGTAATCCAGACCAACTTGCGCGGCAACCACATCGCACTAGTGC210.10532122975073976No Hit
TTTCAGGGGCGACAACTTCGGAGCGCTCTTCCGCTCCCCGAAACAGGGGA210.10532122975073976No Hit
GGATTACCTCATACGGTTGACCTTCCCAAACGCCGGGTTTTTCTTCGAAG210.10532122975073976No Hit
GCGTCCACCTTGGTGCACAACGATTTGCGCCTTGCGCAGGCGGAGGCACG210.10532122975073976No Hit
ATCCAATGCGACTGGTAAACGTGTACCGCCCGGAATCGGCAGTTAGCGAC210.10532122975073976No Hit
GCGTAGTGCTTTGGATGGATGAGCAGGCCCGCGTATACTGGGCCACCGAG210.10532122975073976No Hit
GCCCTGCAAAGCGTAGTGCTTTGGATGGATGAGCAGGCCCGCGTATACTG210.10532122975073976No Hit
CCCATAATCAGGTAATTCAAAGCGAGCCTATTTGGCTTAAACTAACTAGA210.10532122975073976No Hit
GTGATGGTTCCGCGCGCCGGGTTGATCCCGAAAATGCCCTCGGCAACGTT210.10532122975073976No Hit
TGTTAGAACAGCTGGCGTCGGCAGCAGGCGCCCAAGGACAGGATAATGAA210.10532122975073976No Hit
GGCTGATCGAGTTCAAAATTTTGGCACCCGGAATCGCGATGGCGCCAGCG210.10532122975073976No Hit
AACGTGTACCGCCCGGAATCGGCAGTTAGCGACCCTCGCGCTATTGCCTC210.10532122975073976No Hit
GTCCGAAGGCTGATCAGGGGCCTCCCGGCTGGGCTATTAACTGCCGCTGC210.10532122975073976No Hit
GTCCGGCACTGCCAGCGAGGCGCCCAGTGGGCCGTCAACCGAGTTGCCCT210.10532122975073976No Hit
ACTTCGGAGCGCTCTTCCGCTCCCCGAAACAGGGGATGGCGAGCCCATGA210.10532122975073976No Hit
TTTGATACCCAGCTTTTTCACCCCGTAGCGAATAACGCCACGTGCGTCCA200.10030593309594263No Hit
GCGGAGGGCTGGCACTCGGGACGCACGAGCGGATACGCCCCATGCCATGC200.10030593309594263No Hit
GCGCAGGCGGAGGCACGCAAGCGCCAAACCGGACTGACCGTCCGTTTCCA200.10030593309594263No Hit
CTTCCGCTCCCCGAAACAGGGGATGGCGAGCCCATGACCGCCTTGCCAGA200.10030593309594263No Hit
GAACATAATATGTTATGGGAGACTTTCCCGGCAAAAACGAGTTCCCGGAT200.10030593309594263No Hit
GTCCACCTTGGTGCACAACGATTTGCGCCTTGCGCAGGCGGAGGCACGCA200.10030593309594263No Hit
GTATTCTGGCAAGGCGGTCATGGGCTCGCCATCCCCTGTTTCGGGGAGCG200.10030593309594263No Hit
ACCAAAGAGACTACCGGAATCCCGTTCGGCGCTCTGGTGGTAGCTGCGAC200.10030593309594263No Hit
CGCCCATGGCTGGGCGTGGCGCACCGCCGTACCCCATTCCCTATGTCCTC200.10030593309594263No Hit
ATCCAGCTGGGCGTGGCTTGAAATGAATTTTTCAGGGGCGACAACTTCGG200.10030593309594263No Hit
CGTTTCGGGGCTGGCAGGAGGTCCATCGATTGCGCTCGGCGTCCACCTTG200.10030593309594263No Hit
CGCGTATACTGGGCCACCGAGGGCAACGGTAGGGGCCAGGGCGGCGAGGG200.10030593309594263No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)