FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S12_R1_E08-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S12_R1_E08-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences967624
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTTATACCATGAGCATTTTCCCACAATCATGTGCTATCCCTCCCTTAC18100.18705612924028342No Hit
CCCATATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTA11990.12391176738071813No Hit
GTTTTAGATTTGGGCATCCTTTTTTCTACTTTATCTTATACCATGAGCAT10460.10809984043388754No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT10330.10675634337304572No Hit
ATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTATGACA10180.10520615445668978No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)