FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S12_R1_E04-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S12_R1_E04-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234002
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT6990.29871539559490945No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA6260.2675190810334955No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA5110.218374201929898No Hit
AACTCATCCTTTATGACAAAGAAGAAATAAGGAGAGTTTGGCGCCAAGCA5050.2158101212810147No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT4810.20555379868548132No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG4660.19914359706327298No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC4600.19657951641438962No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA4340.18546850026922848No Hit
GTCTTACTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATAT3760.16068238733002282No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG3740.15982769378039505No Hit
GAGTAAGACCTGCTGTTGCATCTTCGCCATTGTTTGCTTGGCGCCAAACT3680.15726361313151171No Hit
CCCATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTA3600.15384483893300058No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC3500.14957137118486166No Hit
AACTTATCCTTTATGACAAAGAAGAAATAAGGAGAGTTTGGCGCCAAGCA3460.1478619840856061No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC3400.14529790343672278No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT3380.144443209887095No Hit
GTGTTGAACCTTGCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGA3360.14358851633746722No Hit
GATTAACTCCATTGCTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGG3310.14145178246339776No Hit
GCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGAACAAGCGCTCTT3280.14016974213895608No Hit
ATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGACA3280.14016974213895608No Hit
GTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCAC3260.1393150485893283No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT3210.13717831471525885No Hit
CTTCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGG3150.13461423406637552No Hit
CTCTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCT3040.12991341954342273No Hit
ATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTATGACA3040.12991341954342273No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGC3030.12948607276860882No Hit
CCCATATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTA2970.1269219921197255No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA2970.1269219921197255No Hit
ACCTAGAAGAGCATCCCAGTGCTGGGAAGGACCCTAAGAAAACAGGAGGA2950.1260672985700977No Hit
TCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATATTGCAC2920.12478525824565602No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT2850.12179383082195878No Hit
CCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAATTTCGGTCATTGATT2810.12008444372270323No Hit
GTTTAACTGCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCA2800.11965709694788933No Hit
TCCCAGCACTGGGATGCTCTTCTAGGTATTTATTTCTTCTCTCATCAAAA2720.11623832274937822No Hit
GCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCG2710.11581097597456431No Hit
GGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGC2690.11495628242493654No Hit
CTCTTACTTGATCCATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCT2640.11281954855086708No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC2640.11281954855086708No Hit
AATGGAGACTGGTGGGGAGCGCCAGGATGCCACAGAAATCAGAGCATCTG2580.11025546790198375No Hit
CCTCAGAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAG2580.11025546790198375No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC2520.10769138725310039No Hit
GTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCTGCGGTGAAAGGA2490.10640934692865874No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT2460.10512730660421706No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTT2440.10427261305458928No Hit
CTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCACCAAACGATCAT2420.1034179195049615No Hit
CTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACT2410.1029905727301476No Hit
CTTGTATAGTTTAACTGCTCTATCCATGTTGTTCGGGTCCCCATTCCCAT2410.1029905727301476No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC2410.1029905727301476No Hit
ATCCAGAATAGCATAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGA2390.10213587918051982No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA2370.10128118563089204No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG2370.10128118563089204No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)