FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S12_R1_C07-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S12_R1_C07-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489848
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA18260.37276869559536835No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT13370.2729418105208146No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC12050.2459946758994627No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA11360.23190867371102875No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA10970.22394702030017472No Hit
CCCATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTA10590.21618951184857343No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG9960.20332837941565546No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC8970.18311802844964153No Hit
ATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGACA8620.1759729548757982No Hit
GAGTAAGACCTGCTGTTGCATCTTCGCCATTGTTTGCTTGGCGCCAAACT8460.17270663552775553No Hit
GCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGAACAAGCGCTCTT8370.17086933089448156No Hit
GTGTTGAACCTTGCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGA8140.16617399683167025No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG8100.16535741699465958No Hit
GTCTTACTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATAT8090.1651532720354069No Hit
GTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCAC7750.15821234342081625No Hit
AACTCATCCTTTATGACAAAGAAGAAATAAGGAGAGTTTGGCGCCAAGCA7470.1524962845617416No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT7450.15208799464323627No Hit
CTCTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCT7350.1500465450507096No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT7320.1494341101729516No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC7250.14800509545818294No Hit
GATTAACTCCATTGCTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGG7190.14678022570266694No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC7060.1441263412323823No Hit
CTTCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGG6790.1386144273325603No Hit
GGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGC6620.135143963025265No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTG6570.13412323822900163No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGC6550.1337149483104963No Hit
ACCTAGAAGAGCATCCCAGTGCTGGGAAGGACCCTAAGAAAACAGGAGGA6490.13249007855498032No Hit
CTCTTACTTGATCCATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCT6370.13004033904394835No Hit
CTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACT6310.12881546928843235No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT6270.12799888945142168No Hit
CCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAATTTCGGTCATTGATT6230.12718230961441102No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAA6210.12677401969590568No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT6190.12636572977740035No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC6160.12575329489964232No Hit
TCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATATTGCAC6130.12514086002188435No Hit
GGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCT6080.124120135225621No Hit
GGATTTATGTGCAACTGATCCCCTCAGAATGAGTGCTGACCGTGCCAGGA6040.12330355538861033No Hit
ATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGA5870.11983309108131503No Hit
GCTTAGTGACACCTCCTTGGCCCCATGGAACGTTATTTCTCTTTTGAGCT5810.11860822132579903No Hit
CTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCACCAAACGATCAT5680.11595433685551437No Hit
GTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCTGCGGTGAAAGGA5650.11534190197775636No Hit
GTTTAACTGCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCA5590.11411703222224037No Hit
GCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCG5390.11003413303718704No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA5350.10921755320017637No Hit
GATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACC5330.10880926328167104No Hit
CTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGGCACCAGACCTTCTG5300.10819682840391305No Hit
CCTCAGAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAG5300.10819682840391305No Hit
TAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAA5290.10799268344466037No Hit
GAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAGCGTTT5240.10697195864839705No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT5230.10676781368914438No Hit
GCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGAT5190.10595123385213372No Hit
TTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCCC5120.10452221913736506No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT5040.10288905946334373No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT4960.1012558997893224No Hit
ATCCAGAATAGCATAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGA4950.10105175483006974No Hit
CAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATAC4900.1000310300338064No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)