FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S12_R1_A06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S12_R1_A06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTCGGGCCATCAGTCATTATAGTAAAACATGAACCATTTACACAGGC27.4074074074074066No Hit
AATTTATAGAGATAAGCAACAAACTGATTGCAGATTTAGAAGTTATCACT13.7037037037037033No Hit
TATAGGAACATGGGCCTCGACACTTGGCCTATCGCAGTCCTCAAGAGCAG13.7037037037037033No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATATAAGAACTAA13.7037037037037033No Hit
CTGTTGCTCTTCTCCCAACCATTGTGAACTCTTCATATCCCTCATGCACT13.7037037037037033No Hit
GTCGTGATGTCGGACGAAATCACAGAATATTCGTAATAGCGAATATTGTA13.7037037037037033No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG13.7037037037037033No Hit
TGCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGCATGAGGAAT13.7037037037037033No Hit
AGTCGAACGGCTAAATCAAGGAACCTTTGGCCCTGTCCATTATAGACACC13.7037037037037033No Hit
CTAGTACTTTACCACATTCGAGACGGATTCAAAAAAAACCGTCAATGAAA13.7037037037037033No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCCTCAACATCCACAGCACACT13.7037037037037033No Hit
GTATTGGCCTCTAATGGCCTTAGGGACTAAAGACTGAAATGGTCCAAATT13.7037037037037033No Hit
GTTTTAAGACAGAGCCCTGTGAAAATGGACAGGCTGCCAAGGCCAATAAA13.7037037037037033No Hit
AGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCCAT13.7037037037037033No Hit
GCTGTATATATCACACAAGCGTTTGGGACAAATTGATGATTTGAACATAA13.7037037037037033No Hit
TCTCAAACACAAGTCTTGAACTGTGACTTTTGGTACTTCATATACAAACA13.7037037037037033No Hit
ATTCAAAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCG13.7037037037037033No Hit
AGCAAATTTTCTGCATAGAACAAGAAGCATGGGTTCTGATGTTAGACAGC13.7037037037037033No Hit
ATAATGGACTATTTCCCCCCGGGGTCCTGATCTTTGAATCATAAGTAATT13.7037037037037033No Hit
GTATCACTATTCACGCCACAAAAAGAAATGCTGCTCGCACTAGTCCAGAT13.7037037037037033No Hit
ACATACTTCAGAGTAAATAGAAATCACAATATTGAGAGAACTACATTTAT13.7037037037037033No Hit
GTTTTGGGGCTCGTGGCTCAGTGTGGATCTGCCCTGTGTGGGCTCATGCT13.7037037037037033No Hit
CTGTTACACTGATACCGGCAAAGTGATGTGTGTGTGCAGAGACAACTGGC13.7037037037037033No Hit
CAAATAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTG13.7037037037037033No Hit
GCTAAGTCTCACACCCATTGCAGCAATCCATATATCCACGGCTTGCTCTA13.7037037037037033No Hit
CATGCACTCTTATCTTCAATGTTTGAACATACCCCGTAAGCACCTCTTCA13.7037037037037033No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)