FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S12_R1_A05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S12_R1_A05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCAAAGTCTACTTTTTCTGGTGCCATATTTTCACCGAGTGCCCACTTC13.7037037037037033No Hit
ATTCTCAGTCTCAATAATTCTGAGCGGCATATCTACATGTACACAGCTAA13.7037037037037033No Hit
ATCCAATTGAATTTGAAACTCAAAAAAAATAACGCACCACAAAAACTAGA13.7037037037037033No Hit
AAGTTAAGCACTGTATTACCCGTCTCCATCCTGAATCTTGGACAAAAGAG13.7037037037037033No Hit
GAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTCCTCTTGGGAATC13.7037037037037033No Hit
TCCTTACACACTTTGAGCATTCACCGCCCACTAGAAGCCCATCTGTGTCA13.7037037037037033No Hit
ATTAACCCACAGATCCACCCAGGCGCTCTTAGACGGTGTATTTTAAAGAA13.7037037037037033No Hit
CCAAATCTCCTGACAACAAACTTTATAACACCAACACTGGATATTACACC13.7037037037037033No Hit
GAGCAATTGGGACAGAAATTTGAGGAAATAAGGTGGTTAATTGAAGAAAT13.7037037037037033No Hit
GCTATAAGCACAACTTTACCGTATACTGGAGCCCCTCCTTACAGCCATGG13.7037037037037033No Hit
GCCTCCAACTGGCAACACTGATTGTTCAAGTTTCTCACAAATACTCCTTG13.7037037037037033No Hit
TGTGTAAATGGTACTTGCTTTACTGTAATGAACGATGGACCAAGTAATGG13.7037037037037033No Hit
GAATCTAATTGCTCCACATTTTCACCATTACTTGTGATTTTCTGTTCCTT13.7037037037037033No Hit
TTATTGCTATTTCCGGCTTGAACTTCTTGCTGTATCTTGATGACCCCACA13.7037037037037033No Hit
TGCCCAAACATATTTTGAATTGGATGCAATCGCTATCATTGAAGAATCCA13.7037037037037033No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT13.7037037037037033No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA13.7037037037037033No Hit
GAGAACAAGAGCGCTTGTTCGCACCGGAATGGATCCCAGAATGTGCTCTC13.7037037037037033No Hit
GGGTAAATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAAT13.7037037037037033No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGGCGAACC13.7037037037037033No Hit
GCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGAACAAGCGCTCTT13.7037037037037033No Hit
TGCCAGGAAAATGAGGTCTTCAATCTCAGCGTTTCCTGGGTTTCGACTTT13.7037037037037033No Hit
GATTGTGAATCCACCCAATCATGAAGGGATTCAAGCCGGAGTCGACAGGG13.7037037037037033No Hit
CCAGTTATCCCTGCACACACATGTGATTTCACTAGAATCAGGATAACAGG13.7037037037037033No Hit
CCAGAACCCGACCTACGCCGCAGCCGCGCACATCAGGATCAATAGCCAGC13.7037037037037033No Hit
GGTCTGAAAGATGACCTTCTTGAAAATTTGCAGGCCTACCAGAAGCGAAT13.7037037037037033No Hit
CCTTAACATATTGAACATGCCCATCATCAATCAAGGCATCAATGATCCAG13.7037037037037033No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)