FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S12_R1_A04-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S12_R1_A04-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGAAGTTGTGGTTTTATAGCAAATCCTTCCCCCCAAAATAACAAAAT22.247191011235955No Hit
CCCAGAGAGAATGACATTAGCTTCCTGCAGGCTCTCATAACATTCTGGAC11.1235955056179776No Hit
AGGGCTTGGCTCTTCCAGCTCCTTGCCAGGCCTTTTTCATCGCACCACAC11.1235955056179776No Hit
GTCGTGAACTTCTCCCAGTAAACAAAGAGTTTATCCATAATACTCTATTA11.1235955056179776No Hit
GCCCTAAATGGGAATGGGGACCCGAACAACATGGATAGAGCAGTTAAACT11.1235955056179776No Hit
GGCCAAGATGAGGAGAAGTGGGAGGAGCCAGTAGAGGGATGACCTCATGG11.1235955056179776No Hit
GAGCAAAAGCAGGTCTGACAGGACCAGAGTGAGGAGGCGGTCTCGCCAGG11.1235955056179776No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC11.1235955056179776No Hit
AAAGAAGAAGAAGTGCTAACGGGCAACCTCCAAACACTGAAAATAAGAGT11.1235955056179776No Hit
GAATAACTTCGCTATGAAGCTGGGTGTACGAATACCTATTTGAGCCCCTG11.1235955056179776No Hit
GATCAGGGCTGCGATCTGATGGGTACAGAGCTGGGGCCCGTCTCCCCAGG11.1235955056179776No Hit
TCCTTAAAAGGAAGAGGCAACACCCTTGGCCTCGATATCGAAACAGCCAC11.1235955056179776No Hit
GTTATATACTGTACTATTTCATTTCTAGAACATTCTCAAAGTGACAACAT11.1235955056179776No Hit
CTGTAGGTTTGCCACCACCTCCTGCATCTTGGGCCGGCTGATGTCCAGGA11.1235955056179776No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC11.1235955056179776No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC11.1235955056179776No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGT11.1235955056179776No Hit
CTCGAACAAAGGTGTAACGGCAGCATGTCCTCATGCTGGAGCAAAAAGCT11.1235955056179776No Hit
CTCATCCAGTTAGGATTAAAGTCTCGAGCGATCTCTTCAAAGTTCCCCTC11.1235955056179776No Hit
GCTGAGTTCAGTAGTCAGAAGTCTGTTGGAGAAGATTTCAGATTGAGTAA11.1235955056179776No Hit
GACCCCAGAGCAGTGCCAACTTGGGCACTACTCACTTTTAATGTGGGTTC11.1235955056179776No Hit
CGGTACACCTGGTCAATGATCTTTTCCTTGACCTGGGAGATGGTGTCACA11.1235955056179776No Hit
GAAGAAGTGCTAACGGGCAACCTCCAAACACTGAAAATAAGAGTACATGA11.1235955056179776No Hit
CTCCAAAGGCATTGTATGGCATTCTTCATAAATGGGCAACAGGAGAAAGA11.1235955056179776No Hit
GTCTTGGTGTATTTCTTTAGTCCAAGATTCAGTAACGACAATACAAATAC11.1235955056179776No Hit
GGAAAACTACAGACTGTGGCCGAGAAGGAAGTGAAGGGAGCCGTGTACTC11.1235955056179776No Hit
CATAATTATAAAACAGCGCACACTAGCCCTCTCCAACTGAGCTGGTCCAG11.1235955056179776No Hit
GCTTTCGCTCCCCCCACCATTGTCTTCTCGGCCATGGGAAGCCTTTCCCT11.1235955056179776No Hit
ACACACGCGCTCCTTTGTGACAACCTATTGGAATCATTTTTGTGTTTCTA11.1235955056179776No Hit
CCACAGCACTCATCCAGTTAGGATTAAAGTCCCGAGCGATCTCTTCAAAG11.1235955056179776No Hit
CTTTGCAGGAAAGAACACAGATCTTGAGGCTCTCATGGAATGGCTAAAGA11.1235955056179776No Hit
AAAATTTCTGTACCAATTGCTCTCGCCACTTTTCATTTCTGCTAACGAGG11.1235955056179776No Hit
GTCTCAAGGTCTCTAATCGGTTAACGATTACACTGAAGTTCGCTTTCAGT11.1235955056179776No Hit
GTATTCAGTCCGTGCTCCGACTCCTGTATGAAGCCATGGGCATTAGCTGC11.1235955056179776No Hit
ATGCAAATGTCTGTTATCTTGTGGTGATGGTTTCAGGGATATATATAGAG11.1235955056179776No Hit
AACATGGACAGACTTGGGTATAATGCTAAGTCAGGTGGAAAAGGCAAACA11.1235955056179776No Hit
GTCTTTTTGTGCATACACACTTGGTTCATTATCCTCAGGCTGTCTCTTAT11.1235955056179776No Hit
CATGTTAGAGGACACCTCTTTATCTGACAGCTCTGGAATAGCTGGTGGAA11.1235955056179776No Hit
GGGGTGGAGGTCTCGCCCAGGTTCTGCATGACCAGAGAGCCGTGACAAAA11.1235955056179776No Hit
GGTTTTGAATAGGCCTGCAGAACTCTGGAGCCCTCGTCCATGTGACCCAT11.1235955056179776No Hit
GGCCAGAGAAAGAGAAGGCACGTAAGTGGGAAAGGAAAAAGCGACACCAT11.1235955056179776No Hit
CCAAAGTCGAGACCGTCAGGGAGGAAGCTGGATAAACACACTCGAACCAC11.1235955056179776No Hit
GGTCAGCTAAAGCCAGAAGTAAATTGTAGTTGAACATGATTTTTGTATAT11.1235955056179776No Hit
TGGCAAGGCAAACACCTCTCCCACCCTCCATCTGCAAGGGGCAGGGCCAT11.1235955056179776No Hit
ACTCCATAATTACAAAGTTGACCACATCAGTATCATTTCTCAAAAGAGTC11.1235955056179776No Hit
CTGCTACTCCATTCCCAAGAAGAAAAGACAAGTACTGCTACTTCCAAAAC11.1235955056179776No Hit
TTTCTTAGGGAAATCTCTCAAGGAGAAAATATCTGGTGACTAGAAATTGT11.1235955056179776No Hit
TCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTCTGCTCTG11.1235955056179776No Hit
GGCCTAGCTTAATCATGCGGTAGATGCGGTTTGAGTGACTCTGGGGATCC11.1235955056179776No Hit
CAAATTTGGCTATGAGGGGCGTAAGCATATGTCTTAATAGCAGGTCATGA11.1235955056179776No Hit
GCCCATTGTGGAGTTGTCCCGAAGGGCCTGGGCTTTCATGATCCGCTCCA11.1235955056179776No Hit
ATGTGGCACATGGTGACCAGCTGGGTCCGACTCTCAAGCCTGGTGTTGTT11.1235955056179776No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG11.1235955056179776No Hit
GATTAGCAACCTCCATGGCCTCCGCTGCCTGTTCACTCGATCCAGCCATC11.1235955056179776No Hit
CCTCATGCTGGAGCAAAAAGCTTCTACAAAAATTTAATATGGCTAGTTAA11.1235955056179776No Hit
TACTGTAGGTTTGCCACCACCTCCTGCATCTTGGGCCGGCTGATGTCCAG11.1235955056179776No Hit
AGCTAGAGGCGGGGTCCTTTGACCTCCAAGTAACTTTTAACAGGCCAGTC11.1235955056179776No Hit
GGATGAGATTTGAACTTGGGCAGTCTGCTCCAGTCCGTGCTATAGTCGGA11.1235955056179776No Hit
GGCTTGGAGTCCCCACAGGCGATGGGTGGGGTGGTAGTGAAAGCTTTTTT11.1235955056179776No Hit
GGAGCAGGCACAGGTGCTGGAGCAGGTGCAGGTGCAGGAGCAGGTGCAGG11.1235955056179776No Hit
GAACACAGATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATCT11.1235955056179776No Hit
CTGATAATGGTTATTTTAGTCCTCATTCTGTTTCATCTAAATGCTTCCAT11.1235955056179776No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGT11.1235955056179776No Hit
ATCCTGGGATCTCTTGACCTGTGCCAAAGGCAGACGCTTGACACTTAACA11.1235955056179776No Hit
GGGTGAGGGGGAGGCCTGCAGGTTCACATGGACGGGAGCTGGGGGCAGAG11.1235955056179776No Hit
TTCATTAGGTGCCAGCTTACGTACAAAGGAATGATTGATCACTTCAAATA11.1235955056179776No Hit
ATGGGACAGCATGGTCCCTGTCATCTGAATCTTCAGACGACTCATCCAGC11.1235955056179776No Hit
GCCAGTTTCCAACAAGACGTGGGCACGAAAACCACCATTCGCCTGATGAA11.1235955056179776No Hit
CTCCTGTACAAGCAGGAGTATGCTTGTACATTCCAGGGTTACAAGGAGCT11.1235955056179776No Hit
AGGGTGGTGGTGGGATTTGCATCAAGGCACTCTCTGGAGTCTTTGAACTT11.1235955056179776No Hit
GGGAGGGAGGCATCAGGAACGGGGCTGTTGGAGGTCCTCCCAGACATGCC11.1235955056179776No Hit
AGGTCACAGTACCTTTTGGAAGACATACAGACATACATTTAAAACTTGTT11.1235955056179776No Hit
CACTAAGCTCAACTCTGAGCTAAGACACAGTAAAGGAGCATCACTCTGGC11.1235955056179776No Hit
CAGCAGGAGCTCTGATATAGTGTCATCTGTCCGGCGACCCATGAGTGACC11.1235955056179776No Hit
TAACATCACAGGCGACAACATTATCAACGTTCCAGCCAACTAATAAACTT11.1235955056179776No Hit
AATATACAGAGATTCGCTTGGAGAAACTGTGATGAGAATGGGAGACCTTC11.1235955056179776No Hit
GGGGAAGGTAGCCCAGAGGAACCCCGGCCCAGACTTCACCCTGTGTCTCT11.1235955056179776No Hit
CTGTTACCAAGGAGGGCCTGGAGCTGCCTGAGGATGAAGAGGAGAAAAAG11.1235955056179776No Hit
ATCATGACCTGAGCTGAAGGCAGATGCTTAACCGACTGAGCTACCCGGGC11.1235955056179776No Hit
TCTTAGAACGGATGCCCCACTGTACCAACTGTGTCTGGGGATATTTAAGA11.1235955056179776No Hit
GAGTGATAGGAATGCCTCATCTTCCAAGGGCACGGACCTCAAGAGCAGCT11.1235955056179776No Hit
CATTTATATAATCAGCTTTCCTATAAAGTCAGTAGGTACACGTGACTGTT11.1235955056179776No Hit
AAATTATGTCCTTTTTACCTAACGGAAAACAAAACATGTTTTGCTCTCTA11.1235955056179776No Hit
CTCCAAACCCTGCTCTTCTTGCCTAAATCAGGCTCTCCCTGCGACCCAGT11.1235955056179776No Hit
GCACCATTATGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAGCCATC11.1235955056179776No Hit
ATATAGCCCATTGTGGAGTTGTCCCGAAGGGCCTGGGCTTTCATGATCCG11.1235955056179776No Hit
CTGCAGCCCCTTGCGGATCCGTTCACGTAACACATACAAGGCAGGGTTTG11.1235955056179776No Hit
ATATTGCACATTCTTTCATAAGCAACCCTTGTCCTTCGTCCATTTTCACA11.1235955056179776No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)