FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S12_R1_A03-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S12_R1_A03-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCAAGAGAGATGCTAATGGGTCTGCTGACACTGCTGCTCTTCTTAC13.125No Hit
GATTGTTTCTGTCCAATTGGATTGAATGAACAAAGAATTGTTGACAGAAG13.125No Hit
ATGTTCCTGGCGATGATTACATATATCACCAGAAATCAACCCGAGTGGTT13.125No Hit
GCACCAGACCTTCTGGGAAGTGTTGAACCTTGCATTAGAGAGCACATTCT13.125No Hit
CTCATGGGATTCCTGTCAGCCCCCAGGGACCGGCTGCCATTGGGAGACCA13.125No Hit
GGTTAACAGTATATGTAACACAGCAGGGGGAGAGAAGCGTTAATTTCTGC13.125No Hit
CCACAAAAGGATATGCTGCTCCCGCTAGTCCACATTGTGTTCTCTTTGGG13.125No Hit
AACAAATAGATAAGCATACAAATCAATCGTTCCTCCCCACGTCCTAGATC13.125No Hit
GTTTGGGTTGGCCCGGGGCTGGTGAATGCGCATGCTTGTGGGAGCGCTTT13.125No Hit
ATACAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAACAGGATG13.125No Hit
GACATATGTTAACATCAGCAACACCAACTTTGCTGCTGGACAGTCAGTGG13.125No Hit
TTTGAGGATATTGCACATTCTTTCATAAGCAACCCTTGTCCTTCGTCCAT13.125No Hit
ATTAAAGACAGGAGCCCATATCGAACCCTAATGAGGTGTCCTATTGGTGA13.125No Hit
GTTTTTTACTCGACCTGTATCGTGACAAAATGACCATCGTCTAAATAACC13.125No Hit
CTACATAGCACGTACCGCCATGCATCCCCTGTTCTGCGAGTAATGTACAA13.125No Hit
GGCCTCAGTTACAACAGGATGGGACCAGTGACCACAGAAGCTGCTTTTGT13.125No Hit
ATATAAAGTAGTAGCCACACTTAAAGAAGTAAAATTTCCAAAGCTGCTAT13.125No Hit
CAAACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCC13.125No Hit
GACGTGGTCAATGTAGGCACAGACCTGACTGGTCTCAGCGTCAGCAACTC13.125No Hit
TATTTATATAGGACTTCTTTTTGCTCATGTTGATTCACACTAACTTGCAG13.125No Hit
TATGAAACTTGATACTCTTAAATCTTCAAATGCAGCAGAGTGGCATGCCA13.125No Hit
GGATTGGTGGTAGTAGCCATCTGTCTGTGAGACCGATGCTGTGAATCAGC13.125No Hit
CATTATAGCTGAGCAGGCCGGCGGCAGCCGATCCAGGACACGGAGCATTC13.125No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT13.125No Hit
CTGTAGGTTTGCCACCACCTACTGCATCTTGGGCCGGCTGATGTCCAGGA13.125No Hit
GTCACAGTTCCAGATGATGGATAATCTGAAATTAATAAAAAAAGCCACAA13.125No Hit
GGAAAGCCACTAATAATTAAATGATAATCACTGTAGTTATTACATGACCT13.125No Hit
GTGCAGGGATAACTGGCATGGCTCGAATCGACCGTGGGTGTCTTTCAACC13.125No Hit
GAACAATAATGAATAGAACAAAGGTTTAACCTCATCATGTACTCATGATT13.125No Hit
CTAGTGGTACCGAGATATGCATTCGCAATGGAAATAAATGCTGGATCTGG13.125No Hit
GTTCAATTTTGGCGTTCACTTCTTTTGACATTTGGGAAAGCTTGCCCTCA13.125No Hit
GGAGAATACTATGAAAGCAGAATCTACTATGCAAACTGAACTGGACGGAT13.125No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)