FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S12_R1_A02-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S12_R1_A02-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAGAAACAAGGTCTTTTTTTCATATATATCGATTGTGAAAAAATTAAAA12.857142857142857No Hit
AGACCTCATTTTCCTGGCACGGTCAGCACTCATTCTGAGGGGATCAGTTG12.857142857142857No Hit
CTTTTGGTCCCCATTTTGCATGTAATGACACGAAAACTAAAGATCTGATC12.857142857142857No Hit
GAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGTAATCT12.857142857142857No Hit
GAAAAGATGAATACACAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCT12.857142857142857No Hit
CGCGACCCCTGCAGAGTCCCACGTCTCCTGTAAAATGGTTCCTCGGAAAG12.857142857142857No Hit
GTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCTGCGGTGAAAGGA12.857142857142857No Hit
GGTGTGCGCACCCCAAGTGTAACCCAACAGCTGATAAAACTTAGCTCCCA12.857142857142857No Hit
CTATAGGCCCTAAGGAGGCCCAGACAGGGGGAGGGAGGTGAGGTGACAGA12.857142857142857No Hit
CCATTTTCTTTTGGTTTTGTCGACTGTTATCTTGATGAAGTCCTAGTAGT12.857142857142857No Hit
AATAATGACTGACAAAGGGATTCAGGGGATAACAAAGTCTTCCACGATAA12.857142857142857No Hit
ATTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTATTCTCTTGTCT12.857142857142857No Hit
CCATCATTCTTATAACTTCTGTTCGCATGTCGGATGTCCGTCCTTCATTG12.857142857142857No Hit
ACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTT12.857142857142857No Hit
CTTAATAAGTCTTCAAACACCTTGATTAGGATATGCATCACACACACACA12.857142857142857No Hit
CACTTGAATCGCTGCATCTGCACTCCCATTCGCTTCTGGTAGGCCTGCAA12.857142857142857No Hit
GCCCCAAACTCTCCTTATTTCTTCTTTGTAATAAAGGATCAGTTCTCTCA12.857142857142857No Hit
ATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATTCAGATGCATATG12.857142857142857No Hit
ATTAAAGACAGGAGCCCATATCGAACCCTAATGAGCTGTCCTATTGGTGA12.857142857142857No Hit
GTATTATTGTTAGAGTATAATGTCTTAAAATTTCTAAAAATTGAATCCTC12.857142857142857No Hit
CTGAGACATATTACAACAAAACTTTCAAAAGACTAAAACAGAATCTTGAA12.857142857142857No Hit
GCCCAGGGTGGGGGTGCCCTGACATGTGCGTTCGGAGTTGCCTTCCCGCG12.857142857142857No Hit
GAATCAATGACCGAAATTTCTGGGGGGGTGAAAATGGACGAAGGACAAGG12.857142857142857No Hit
CCTGTAGAATCTGTCCACTCCTGCTTGTATTCCCTCATGGTTTGGTGCAT12.857142857142857No Hit
GGGAAGGAGCAATCCACAGGATTAGAGTTCTGTTCTACAACTCCCCAGAG12.857142857142857No Hit
CAACAAAAATGAAGGCAATACTAGAAGTTCTGCTATATACATTTGCAACC12.857142857142857No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC12.857142857142857No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC12.857142857142857No Hit
ATATACTAAATTAATTTCAAGTAATACCATTTACAGGACCAAGAAGAAAG12.857142857142857No Hit
GGGCAGGCTGCCCGCAGCTCTGTTTGTTTTCCTGCAGTCTGAGGCTGGGG12.857142857142857No Hit
TATAAGTACAGCAGACTCCCACCAAGAAGTGCATTAATATTTCTCTTCAG12.857142857142857No Hit
ACAGTTTCCTACACCTGACCTCCTCTGTGTCTGGGTCCCATGTTGGGGGG12.857142857142857No Hit
ACTTGATACTCTTAAATCTTCAAATGCAGCAGAGTGGCATGCCATCCACA12.857142857142857No Hit
TAACTATATTTAAAATGCTGGCATGCACCATACAGTCAGCTCTCAATTTC12.857142857142857No Hit
CTATGAAAGGGGCTAGTGGCTTTTATCCAATGAGGCATACTTGTATCATC12.857142857142857No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)