FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S12_R1_A01-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S12_R1_A01-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC13.0303030303030303No Hit
GTTTGGCGCCAAGCAAACAATGGCGAAGATGCAACAGCAGGTCTTACTCA13.0303030303030303No Hit
AATGTGAAGATTCCGTATATTGTATAAGTTTGGTCCTCCATCTGATACTA13.0303030303030303No Hit
GCTAGCACCTGCTTCCAAGCCATGAGGTAATTGGGATTTATGCCTTTCTC13.0303030303030303No Hit
ACTATACCATCTCACTATTACAGAAGCTCAACATAATTTAAACACAACTC13.0303030303030303No Hit
AGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAA13.0303030303030303No Hit
CCATTACATTCTCTTCCAGGACATACTTATGAGGATGTCAATAATGCAGT13.0303030303030303No Hit
GTAGTGGTGAAATTTCTCCAACTATTGCTCCCTCCTCAGTGAAAGCCCTT13.0303030303030303No Hit
GTCATTAACTAAAAAGAACAGAGGGTTGCATTTTTCTTGTAACCATTCAG13.0303030303030303No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGTAGCATTTGCGAAAAGCTTGA13.0303030303030303No Hit
GTATTAGAAAGAAATTGACTGTAACTGGGTTTCAAGTGAAATTTGGAGAA13.0303030303030303No Hit
GATCGTCATTATCTCAGAGAAATATTCTATCTTGATAAGTCCAGAATCGA13.0303030303030303No Hit
CTCGGACAACCAATCGGTGTCCTACAATTCAACTCAACTCTAATTGTATC13.0303030303030303No Hit
CAATAAAAGGAATAAACCAGACTAAGTTTGATAATTGTAACTGGCAAAAG13.0303030303030303No Hit
ATACTAGGGGCTGTCACTGAGGAGGGAGCAATAGTTGGAGAAAGTTCAAT13.0303030303030303No Hit
CTAAAAATATTTTTTAAGAAAAGATTTCACAGGGGAATTTTTAAAATACA13.0303030303030303No Hit
AATAAGGCCTCTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGA13.0303030303030303No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTT13.0303030303030303No Hit
TTGCTAATCAGACTAGGCAGATGGTACATGCAATGAGAACTATTGGGACT13.0303030303030303No Hit
GGAGAAAACAAGTCCTAGTAAGGATGTGGACGTTGGTGGGCACATAAAAT13.0303030303030303No Hit
AACTAGGACCTGAGACAGTAGCTTCCTCTGCCCATGGGGTCATCCCCCTG13.0303030303030303No Hit
CTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGAACACAAATCCTAAAA13.0303030303030303No Hit
CTCACATTCACAGTCAATGAGGAAAATTGCATCCTACTCTGTTCTGGTGG13.0303030303030303No Hit
ATATAACACATAATTCATATATTTGTGACATTCTCCCATGAGATTGAGAG13.0303030303030303No Hit
ACCAAGGAATTATTTGAAAACAAATCGGAAACATCGCCAATCGGAGATTC13.0303030303030303No Hit
CCCCTGTTGAAGGAGATCAGATCTAATTTCTCCTCCGCGGAGACCACCGA13.0303030303030303No Hit
GAAATCAAGTGTCCACGGCAGAAGGTACAACCTCAGGGATTTTTTTTAAG13.0303030303030303No Hit
ATCTTGATGACCCCACAAAAACATATGCATCTGCATTCTGATAGAGACTT13.0303030303030303No Hit
GGCCTGCGGATGCCTTTTGTTGATTGGTATTTCCTCCACTCCTGGTCCTT13.0303030303030303No Hit
GCAGCTGCTGATCCCTTAGCGCGGAGGGGGAAAGGCAGGAGAACTAGAAA13.0303030303030303No Hit
GACTAGAACCAAGAGCAGACACTTAATGACTGAGCCACCCAGGCGCCCCA13.0303030303030303No Hit
TCAATGGCCCTGAGTCAGTGCTAGTCAACACTTATCAATGGATAATCAGG13.0303030303030303No Hit
CCATGGAGAATATTTCATCGCAATAAAACACCCTATGATTTTCACATTGG13.0303030303030303No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)