FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S11_R1_H11-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S11_R1_H11-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887680
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG75980.855939077144917No Hit
GTACTATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAG37820.4260544340302812No Hit
GTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACCCTGCAAAGGAA25800.2906452775775054No Hit
GTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATGAACAAGGAATAACC24420.2750991348233598No Hit
CTGTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACCCTGCAAAGG18080.20367700072098055No Hit
CTATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAA13200.1487022350396539No Hit
TTTCAAATACTGTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACC11690.13169160057678445No Hit
GTTTTATAGCATTATTCAAGCCTTTAAAGTTAGCAGTTAAATGATGATCT11360.12797404470079307No Hit
ATTTAGTATTTCAGACCCAATCTTCTCCCCAACCCTGCAAAGGAAACCAA11150.12560832732516222No Hit
GCATTATTCAAGCCTTTAAAGTTAGCAGTTAAATGATGATCTAAATCTTC10830.12200342465753425No Hit
ATTCAAGCCTTTAAAGTTAGCAGTTAAATGATGATCTAAATCTTCAGAAG10690.12042627974044702No Hit
ATATATGCCAAACAAACTTCTATTTTCTAAAGACTCTGGGATTGCTAAGT10030.11299116798846431No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)