FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S11_R1_G11-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S11_R1_G11-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841686
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAGGATTGCCTGTGAGGCCAATGCCTGGGATGTGCAGGCAGCACCT17560.20862887109919853No Hit
GCTTAGGAATGATGAGTGTGATTCTCTTCAGGCCCTGAGCTGTGTCATCC15340.1822532393315322No Hit
GTACTGACTCTGTGGCTTCTGCCTGATGCTCAGCATCGACTGATGTGGGC14050.16692685870978013No Hit
TAATAATACAGGTTGTAAGTAGCAAAACCAGTATTTAAGCACCATCCATT12500.14851144013325634No Hit
TTTCTATACCTCAGCCTTCTTTTCCACAGTCTTTCTAGGTCTTGTCTAAT12130.14411550150531194No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA11820.1404324177900072No Hit
TGTTACTACTGATGCCCTGGGGAGAAGCTGTTAGCCTCTCCTGTAGGTTC11350.13484838764099674No Hit
ATCCTGAGCACTGTGGGGGAAGAACAGGTTCCGGGAGAGGTACTAGGATT10750.12771983851460045No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG10660.126650556145641No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT10350.12296747243033626No Hit
ATACAGGAGAGCTCCCTACAACAAAAAGTTCCTTGGTCCAAAATACCAGT9090.10799751926490402No Hit
CTTCAGGCCCTGAGCTGTGTCATCCAGGCACCTTCCCAGAGCATTTAGGT8820.10478967215802566No Hit
CTTTATATGAGCCCCTTGGAGCAGAACAAGACCATCATTGTGGGCAGAGG8800.10455205385381247No Hit
TTACTATACATTGGTTTATTAGGACAAGGTTTCTCATCTTCAGCACTACT8650.10276991657221339No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)