FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S11_R1_D10-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S11_R1_D10-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834687
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA23570.28238129981657795No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT23460.2810634405471752No Hit
GTCTTACTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATAT21900.2623737999992812No Hit
CTCTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCT20150.24140785707696416No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA19890.2382929169856485No Hit
CCCATATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTA19130.22918770748795655No Hit
GTGTTGAACCTTGCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGA17820.21349320164325072No Hit
GCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGAACAAGCGCTCTT17550.21025845616380753No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA17550.21025845616380753No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC17470.20930001305878732No Hit
CCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAATTTCGGTCATTGATT17280.20702371068436431No Hit
GAGTAAGACCTGCTGTTGCATCTTCGCCATTGTTTGCTTGGCGCCAAACT17070.20450779753368625No Hit
GGATTTATGTGCAACTGATCCCCTCAGAATGAGTGCTGACCGTGCCAGGA16920.2027107167117734No Hit
GATTAACTCCATTGCTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGG16380.196241225752887No Hit
CTCTTACTTGATCCATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCT16240.19456395031910165No Hit
GTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCTGCGGTGAAAGGA16040.19216784255655114No Hit
GTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCAC15630.18725582164332258No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATG15430.18485971388077208No Hit
CCCATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTA14740.1765931420999728No Hit
TCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATATTGCAC14700.1761139205474627No Hit
ATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTATGACA14300.17132170502236166No Hit
GTGCTGGGAAGGACCCTAAGAAAACAGGAGGACCCATATATAGAAGAGTA13640.163414549405945No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC13210.1582629177164614No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC13070.15658564228267602No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT13030.15610642073016592No Hit
ACCTAGAAGAGCATCCCAGTGCTGGGAAGGACCCTAAGAAAACAGGAGGA12940.15502817223701817No Hit
CTTCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGG12650.15155381598131995No Hit
GGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGC12630.1513142052050649No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT12530.15011615132378964No Hit
CTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCACCAAACGATCAT12470.14939731899502448No Hit
GAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAGCGTTT12090.1448447142461785No Hit
CCTCAGAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAG12090.1448447142461785No Hit
AGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAACAGGC11840.14184957954299038No Hit
CTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGGCACCAGACCTTCTG11840.14184957954299038No Hit
TATCATGATTTGGCATTCCAACCTGAATGATGCCACATATCAGAGAACAA11710.14029210949733253No Hit
TCCCAGCACTGGGATGCTCTTCTAGGTATTTATTTCTTCTCTCATCAAAA11590.13885444483980222No Hit
ATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGACA11500.1377761963466545No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGC11480.13753658557039944No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG11240.13466125625533884No Hit
CTTCTAGGTATTTATTTCTTCTCTCATCAAAAGCAGAAAGCACCATCCTC11150.1335830077621911No Hit
ATATTGCACATTCTTTCATAAGCAACCCTTGTCCTTCGTCCATTTTCACC11010.13190573232840572No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC10740.12867098684896255No Hit
CTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACT10580.12675410063892212No Hit
ATCCAGAATAGCATAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGA10550.12639468447453955No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC10480.1255560467576469No Hit
AATGGAGACTGGTGGGGAGCGCCAGGATGCCACAGAAATCAGAGCATCTG10460.12531643598139183No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG10070.12064402584441832No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA10040.12028460968003575No Hit
TTCTTTCATAAGCAACCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAA10040.12028460968003575No Hit
GTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAGCGTTTCCTGGG10000.11980538812752564No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT9970.11944597196314308No Hit
GTTCCAACTCCTTTCACCGCAGCACCTGCGGCACCAGACCTTCTGGGAAG9860.11812811269374028No Hit
CCTCTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATATTGCACATTCTTT9760.11693005881246504No Hit
ATATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTATGA9740.11669044803620998No Hit
TCCCAGTGCTGGGAAGGACCCTAAGAAAACAGGAGGACCCATATATAGAA9730.11657064264808245No Hit
ATCCATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATAT9690.11609142109557234No Hit
ATGTGCAACTGATCCCCTCAGAATGAGTGCTGACCGTGCCAGGAAAATGA9500.11381511872114937No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT9370.11225764867549154No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAA9360.11213784328736401No Hit
TTCATAAGCAACCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAATTTC9240.1107001786298337No Hit
GGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCT9000.10782484931477307No Hit
CTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCTGC8950.10722582237413546No Hit
GAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGA8930.1069862115978804No Hit
GTTTAACTGCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCA8740.10470990922345742No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT8660.1037514661184372No Hit
GATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCACCAA8550.10243360684903444No Hit
TCCCAGAAGGTCTGGTGCCGCAGGTGCTGCGGTGAAAGGAGTTGGAACAA8470.10147516374401423No Hit
GAACAAGAGCGCTTGTTCGCACCGGAATGGATCCCAGAATGTGCTCTCTA8390.10051672063899403No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)