FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S11_R1_D04-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S11_R1_D04-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195922
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG35970.3007721239345041No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA34940.29215952210930146No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC28860.24132008609257125No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT27040.22610170228493162No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT25490.21314099080040338No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA25300.21155225842488054No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT22670.1895608576479068No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA20940.1750950312813043No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC20260.16940904172680157No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT19500.1630541122247103No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC19440.1625524072640189No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT18460.15435789290605909No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG18380.15368895295847054No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC17910.14975893076638777No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA17460.14599614356120216No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTT17310.14474188115947362No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG14350.11999110309869707No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT13980.1168972558411No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC13920.11639555088040858No Hit
CCCATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTA13790.11530852346557718No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC13700.11455596602454006No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT13670.11430511354419434No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG13580.11355255610315722No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA13580.11355255610315722No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG13570.11346893860970865No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC13540.11321808612936295No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT13490.1127999986621201No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC13210.11045870884556015No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA13080.10937168143072876No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC12900.10786656654865451No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGAC12600.10535804174519744No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT12570.10510718926485171No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG12370.10343483939588033No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC12260.10251504696794607No Hit
ATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGACA12240.10234781198104895No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT12170.10176248952690894No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)