FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S11_R1_C12-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S11_R1_C12-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences940644
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG20630.2193178290617917No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG19320.2053912000714404No Hit
CTATAGTACTGGTCATACCTATTTTACGAATAGAGAAATGAAGTTTACTT18090.19231505224080522No Hit
GTATGATACATGGGGAGGCTCCTTAGGGAGAGTAAGAATTAAATGAACAC12540.13331292178550017No Hit
TTCATAAAGTGATTCATTTGTTAGGAACATACAGCCATCCAGACGCAGTG11150.11853581163543275No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT10570.11236982322749096No Hit
GTACTATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAG9510.10110094786125251No Hit
GAGTAGAGAATGAGTAGGACTGAGCTAAATAAAAGGATGGCAGTGGGGTG9480.10078201742635896No Hit
CTATTAATATAGGGCAACATTTCCCAAAGCGTGGTATGCTGAACGCTGAC9430.1002504667015364No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)