FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n02_S11_R1_A11-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n02_S11_R1_A11-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887651
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAATAGTGGCCGGATTTTTTCAATTTTCTTTCTTGTTGAATCATTG17820.2007545758411808No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAATGCATGTGTT17060.1921926522923987No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT15890.17901179630282624No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC14430.16256389053806058No Hit
GTATGGAGAGGATCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA12850.14476410210769775No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAATCC12300.13856797322371067No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCATTTCATCAATGAGCA10840.122120067458945No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC10720.12076818479334783No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA10710.12065552790454807No Hit
ATTCAATAGTCTGTATGCATCTCCACAATTAGAAGGATTTTCAGCTGAAT10470.11795176257335371No Hit
GTCATAAGTTCCATTTTTCACACTTTCCATGCATTCATTGTTACACTTGT10260.11558596790855866No Hit
ATATAATGCAGAATTGTTGGTTCTACTGGAAAATGAAAGGACTTTGGATT10150.11434674213176126No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAA9390.10578481858297911No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA9360.10544684791657982No Hit
GTTTGTATACTTTGTTGAGACACTGGCAAGAAGTATATGTGAAAAACTTG9290.10465824969498147No Hit
GGTTCAGATTGTCCCTAAGAGCCTGAACAATAAGGAGCAATTTTCTTGAT9210.10375699458458336No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC8950.10082791547578948No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)