FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S17_R1_H08-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S17_R1_H08-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914090
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAACACAATGTAACACAATGCTTGACTTCAGGGGAAATACTCAACCA18350.20074609721143435No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT15340.16781717336367316No Hit
ATGTAACACAATGCTTGACTTCAGGGGAAATACTCAACCAAAAAATCTAG13970.1528295900841274No Hit
ATGTAACACAATGTAACACAATGTAACACAATGCTTGACTTCAGGGGAAA12370.1353258431883075No Hit
GTGTTACATTGTGTTACATTGGGCCTTACCTCATCTGGTCTTATCAGCAG12200.13346607008062664No Hit
TCCCCGAAGTAACTCATTCACCCGTTAAATAAATAGTGATCCAACATCAA10540.1153059326762135No Hit
CTTTAGCGAGTCTCATCGGCTGCAAATCCATAAAACACCCTGCACTTCCA10170.11125819120655517No Hit
GTGTTACATTGGGCCTTACCTCATCTGGTCTTATCAGCAGGGCTGTGAAA10020.10961721493507204No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA9900.10830443391788554No Hit
CCACAGGGCCAGTCCCTGAGTCTGTCACATACACAGCAATACAAGCTTCT9700.10611646555590806No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG9580.10480368453872158No Hit
TCTTATATGTTCTTGATTTTAAGACAAAGGCTGCATGATTTGAGCTAATG9570.10469428612062269No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA9420.10305330984913957No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG9420.10305330984913957No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA9190.10053714623286547No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)