FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S17_R1_H04-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S17_R1_H04-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616049
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA27870.4523990786447182No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA20430.3316294645393467No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA17990.2920222255047894No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC15420.2503047647183909No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA15100.2451103727138588No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC14480.23504623820507783No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT13250.21508029393765754No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA12980.2106975256838336No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA11280.18310231815975678No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC11230.18229069440904863No Hit
GTGTAATTTTTATCTTTATTTCTCTGTATCTACTGTATCTTACTTTTTCC11140.18082977165777397No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC10530.17092796189913464No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT10180.16524659564417765No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG8900.14446902762604924No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG8310.13489186736769315No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG8160.13245699611556871No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA7660.12434075860848731No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA7640.12401610910820406No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT7500.12174356260622125No Hit
AAATTACACTGGCCATTCACCAGAAAAAAAATGAACTCAAGCCCAACCAT7490.12158123785607963No Hit
TCCCAGGACTTTGGAATCATGACCTGAGCTGAAGGCAGACACTTAACAAC7430.12060728935522987No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA7360.11947101610423846No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA7220.11719846960225568No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT7160.1162245211014059No Hit
CATCATGGATTTTCTTTATTATTATTTTCAGCAATTCAAGTGTGATATGT6920.11232872709800681No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA6750.10956920634559912No Hit
CCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAACTATCCATACAAA6740.10940688159545751No Hit
GTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATT6730.10924455684531587No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA6660.10810828359432446No Hit
CTTTAAAACACTTATTATGAATAAGGATAAGAATATAAAAAATACGACCA6580.10680968559319144No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG6540.10616038659262494No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA6300.10226459258922586No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)